Akt (phospho Ser473) Polyclonal Antibody
- Catalog No.:YP0006
- Applications:IF;WB;IHC;ELISA
- Reactivity:Human;Mouse;Rat;Chicken
- Target:
- AKT1/2/3
- Fields:
- >>EGFR tyrosine kinase inhibitor resistance;>>Endocrine resistance;>>Platinum drug resistance;>>MAPK signaling pathway;>>ErbB signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>cGMP-PKG signaling pathway;>>cAMP signaling pathway;>>Chemokine signaling pathway;>>HIF-1 signaling pathway;>>FoxO signaling pathway;>>Sphingolipid signaling pathway;>>Phospholipase D signaling pathway;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Apoptosis;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Cellular senescence;>>Adrenergic signaling in cardiomyocytes;>>VEGF signaling pathway;>>Apelin signaling pathway;>>Osteoclast differentiation;>>Focal adhesion;>>Signaling pathways regulating pluripotency of stem cells;>>Platelet activation;>>Neutrophil extracellular trap formation;>>Toll-like receptor signaling pathway;>>C-type lectin receptor signaling pathway;>>JAK-STAT signaling pathway;>>T cell recept
- Gene Name:
- AKT1/AKT2/AKT3
- Protein Name:
- RAC-alpha serine/threonine-protein kinase/RAC-beta serine/threonine-protein kinase/RAC-gamma serine/threonine-protein kinase
- Human Gene Id:
- 207/208/10000
- Human Swiss Prot No:
- P31749/P31751/Q9Y243
- Mouse Gene Id:
- 11651/11652/23797
- Rat Gene Id:
- 24185/25233/29414
- Rat Swiss Prot No:
- P47196/P47197/Q63484
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human Akt around the phosphorylation site of Ser473. AA range:431-480
- Specificity:
- Phospho-Akt (S473) Polyclonal Antibody detects endogenous levels of Akt protein only when phosphorylated at S473.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- AKT1;PKB;RAC;RAC-alpha serine/threonine-protein kinase;Protein kinase B;PKB;Protein kinase B alpha;PKB alpha;Proto-oncogene c-Akt;RAC-PK-alpha;AKT2;RAC-beta serine/threonine-protein kinase;Protein kinase Akt-2;Protein kinase B
- Observed Band(KD):
- 56kD
- Background:
- The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
- Function:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Defects in AKT1 are associated with breast cancer (BC) [MIM:114480]. BC is an extremely common malignancy, affecting one in eight women during their lifetime.,disease:Defects in AKT1 are associated with colorectal cancer (CRC) [MIM:114500].,disease:Defects in AKT1 are associated with susceptibility to ovarian cancer [MIM:604370]; also called susceptibility to familial breast-ovarian cancer type 1 (BROVCA1).,domain:Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.,domain:The AGC-kinase C-terminal mediates interaction with THEM4.,enzyme regulation:Three specific sites, one in the kinase domain (Thr-308) and the two other ones in the C-terminal regulatory region (Ser-473 and Tyr-474), need to be phosphorylated for its full activation.,function:Gene
- Subcellular Location:
- Cytoplasm . Nucleus . Cell membrane . Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. Colocalizes with WDFY2 in intracellular vesicles (PubMed:16792529). .
- Expression:
- Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
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HSP90 inhibitor AUY922 can reverse Fulvestrant induced feedback reaction in human breast cancer cells. CANCER SCIENCE 2017 May 19 WB Human MCF-7 cell,T47D cell
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Hydroxy-α-sanshool Possesses Protective Potentials on H2O2-Stimulated PC12 Cells by Suppression of Oxidative Stress-Induced Apoptosis through Regulation of PI3K/Akt Signal Pathway. Oxidative Medicine and Cellular Longevity Oxid Med Cell Longev. 2020;2020:3481758 WB Rat 1 : 1000 PC12 cell
Autophagy was activated against the damages of placentas caused by nano-copper oral exposure. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Ecotox Environ Safe. 2021 Sep;220:112364 WB Rat 1 : 1000 Placentas
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Cadmium induces BNIP3-dependent autophagy in chicken spleen by modulating miR-33-AMPK axis. CHEMOSPHERE 2017 Dec 06 WB Chicken 1:500 Spleen
The PI3K/p-Akt signaling pathway participates in calcitriol ameliorating podocyte injury in DN rats. METABOLISM-CLINICAL AND EXPERIMENTAL Metabolism. 2014 Oct;63:1324 WB Rat Renal tissues
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Cardamonin inhibits angiotensin II-induced vascular smooth muscle cell proliferation and migration by downregulating p38 MAPK, Akt, and ERK phosphorylation. Journal of Natural Medicines J Nat Med-Tokyo. 2014 Jul;68(3):623-629 WB Rat VSMCs
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Tian, Sha-Sha, et al. "Flavonoids from the leaves of Carya cathayensis Sarg. inhibit vascular endothelial growth factor-induced angiogenesis." Fitoterapia 92 (2014): 34-40.
Wang, Wei-wei, et al. "Telmisartan reduces atrial arrhythmia susceptibility through the regulation of RAS–ERK and PI3K–Akt–eNOS pathways in spontaneously hypertensive rats." Canadian journal of physiology and pharmacology 93.8 (2015): 657-665.
Cao, Zheng, et al. "Exendin-4 attenuates high glucose-induced endothelial progenitor cells dysfunction through the PI3-K/Akt/eNOS pathway." INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 10.6 (2017): 6774-6780.
Zhang, Xianxie, et al. "Salvia miltiorrhiza alleviates hypoxia-induced nerve injury associated with Ngb/Akt intracellular signaling pathway." (2020).
刘素娟, et al. "染料木素通过 TIPE 2 负性调控 Akt 活性促进脂多糖活化的巨噬细胞凋亡." (2020).
佟雪, et al. "基于 PI3K/AKT/mTOR 信号通路研究补肾法对体外培养小鼠卵母细胞成熟的促进作用." 北京中医药大学学报 44.6 (2021): 510-518.
恪雪, et al. "基于 PI3K/AKT/mTOR 信号通路研究补肾法对体外培养 小鼠卵母细胞成熟的促进作." Journal of Beijing University of Traditional Chinese Medicine 44.6 (2021).
Cadmium induces BNIP3-dependent autophagy in chicken spleen by modulating miR-33-AMPK axis. CHEMOSPHERE 2017 Dec 06 WB Chicken 1:1000 Spleen
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CB2R agonist GW405833 alleviates acute liver failure in mice via inhibiting HIF-1α-mediated reprogramming of glycometabolism and macrophage proliferation ACTA PHARMACOLOGICA SINICA Ze-bing Huang WB Mouse RAW264.7 cells
Sec61γ is a vital protein in the endoplasmic reticulum membrane promoting tumor metastasis and invasion in lung adenocarcinoma BRITISH JOURNAL OF CANCER Lishuang Qi WB Human,Mouse lung cancer A549 cell,H1650 cell
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HMGB1 released from dead tumor cells after insufficient radiofrequency ablation promotes progression of HCC residual tumor via ERK1/2 pathway INTERNATIONAL JOURNAL OF HYPERTHERMIA Baoming Luo WB Human HepG2 cell,Huh7 cell
UQCRFS1 serves as a prognostic biomarker and promotes the progression of ovarian cancer. Jing Wang WB Human 1:1000 ovarian tissue A2780 cell,OVCAR8 cell
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CXADR promote epithelial–mesenchymal transition in endometriosis by modulating AKT/GSK-3β signaling CELL CYCLE Hang-Jing Tan WB Human 1:1000 Ishikawa cell
Roflupram alleviates autophagy defects and reduces mutant hSOD1-induced motor neuron damage in cell and mouse models of amyotrophic lateral sclerosis NEUROPHARMACOLOGY Di Huo WB Mouse 1:1000 spinal cord NSC34 cell
Inhibitor PF-04691502 works as a senolytic to regulate cellular senescence EXPERIMENTAL GERONTOLOGY Ziqiang Fan WB Mouse 1:500 NIH-3T3 cell
The gut microbiota contributes to the infection of bovine viral diarrhea virus in mice JOURNAL OF VIROLOGY Zecai Zhang WB Mouse peripheral blood lymphocytes (PBL)
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MiR-21-5p ameliorates Busulfan-induced testicular dysfunction and maintains spermatogenesis1 Journal of Integrative Agriculture Meng-fei ZHANG WB Goat Male germ stem cells (mGSCs)
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The FGF6 amplification mutation plays an important role in the progression and treatment of malignant meningioma Translational Oncology Ruihao Liang WB Human IOMM-LEE cell,CH157MN cell
Dexmedetomidine improves the circulatory dysfunction of the glymphatic system induced by sevoflurane through the PI3K/AKT/ΔFosB/AQP4 pathway in young mice Cell Death & Disease Wang Shuying WB Mouse 1:2000 hippocampus tissue primary astrocytes
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- Products Images
- Ge, Lu, et al. "PRMT5 promotes epithelial‐mesenchymal transition via EGFR‐β‐catenin axis in pancreatic cancer cells." Journal of cellular and molecular medicine 24.2 (2020): 1969-1979.
- Guo, Moran, et al. "Deletion of FGF9 in GABAergic neurons causes epilepsy." Cell death & disease 12.2 (2021): 1-13.
- Qin, Qin, et al. "miR‐134 inhibits non‐small cell lung cancer growth by targeting the epidermal growth factor receptor." Journal of cellular and molecular medicine 20.10 (2016): 1974-1983.
- Xiong, Yang, et al. "CCL21/CCR7 interaction promotes cellular migration and invasion via modulation of the MEK/ERK1/2 signaling pathway and correlates with lymphatic metastatic spread and poor prognosis in urinary bladder cancer." International journal of oncology 51.1 (2017): 75-90.
- Wang, Xinzhao, et al. "Ad-p53 enhances the sensitivity of triple-negative breast cancer MDA-MB-468 cells to the EGFR inhibitor gefitinib." Oncology reports 33.2 (2015): 526-532.
- Liu, Jianghua, et al. "MicroRNA-32 promotes calcification in vascular smooth muscle cells: Implications as a novel marker for coronary artery calcification." PloS one 12.3 (2017): e0174138.
- Fan, Hua, et al. "Scutellarin Prevents Nonalcoholic Fatty Liver Disease (NAFLD) and Hyperlipidemia via PI3K/AKT-Dependent Activation of Nuclear Factor (Erythroid-Derived 2)-Like 2 (Nrf2) in Rats." Medical science monitor: international medical journal of experimental and clinical research 23 (2017): 5599.
- Immunofluorescence analysis of rat-liver tissue. 1,Akt (phospho Ser473) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
- Immunofluorescence analysis of rat-liver tissue. 1,Akt (phospho Ser473) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
- Western Blot analysis of 3T3 cells treated with PDGF using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)
- Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,Akt (phospho Ser473) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
- Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,Akt (phospho Ser473) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
- Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,Akt (phospho Ser473) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
- Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Akt (phospho Ser473) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
- Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,Akt (phospho Ser473) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
- Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue. 1,Akt (phospho Ser473) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
- Immunohistochemical analysis of paraffin-embedded Rat-brain tissue. 1,Akt (phospho Ser473) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
- Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,Akt (phospho Ser473) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
- The picture was kindly provided by our customer
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Akt (Phospho-Ser473) Antibody. The picture on the right is blocked with the phospho peptide.
- Western blot analysis of lysates from HeLa cells treated with heat shock, using Akt (Phospho-Ser473) Antibody. The lane on the left is blocked with the phospho peptide.