• Target：
• FoxO1

• Fields：
• >>FoxO signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Cellular senescence;>>Insulin signaling pathway;>>Thyroid hormone signaling pathway;>>Glucagon signaling pathway;>>Insulin resistance;>>AGE-RAGE signaling pathway in diabetic complications;>>Alcoholic liver disease;>>Shigellosis;>>Human papillomavirus infection;>>Pathways in cancer;>>Transcriptional misregulation in cancer;>>Prostate cancer

• Gene Name：
• FOXO1

• Protein Name：
• Forkhead box protein O1

• Human Gene Id：
• 2308

• Human Swiss Prot No：
• Q12778

• Mouse Gene Id：
• 56458

• Mouse Swiss Prot No：
• Q9R1E0

• Rat Gene Id：
• 84482

• Rat Swiss Prot No：
• G3V7R4

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human FKHR around the phosphorylation site of Ser256. AA range:223-272

• Specificity：
• Phospho-FoxO1 (S256) Polyclonal Antibody detects endogenous levels of FoxO1 protein only when phosphorylated at S256.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• FOXO1;FKHR;FOXO1A;Forkhead box protein O1;Forkhead box protein O1A;Forkhead in rhabdomyosarcoma

• Observed Band(KD)：
• 82kD

• Background：
• This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008],

• Function：
• disease:Chromosomal aberrations involving FOXO1 are a cause of rhabdomyosarcoma 2 (RMS2) [MIM:268220]; also known as alveolar rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.,function:Transcription factor.,PTM:Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 fork-head DNA-binding domain.,subcellular location:Shuttles betw

• Subcellular Location：
• Cytoplasm . Nucleus . Shuttles between the cytoplasm and nucleus. Largely nuclear in unstimulated cells (PubMed:11311120, PubMed:12228231, PubMed:19221179, PubMed:21245099, PubMed:20543840, PubMed:25009184). In osteoblasts, colocalizes with ATF4 and RUNX2 in the nucleus (By similarity). Serum deprivation increases localization to the nucleus, leading to activate expression of SOX9 and subsequent chondrogenesis (By similarity). Insulin-induced phosphorylation at Ser-256 by PKB/AKT1 leads, via stimulation of Thr-24 phosphorylation, to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway (PubMed:11237865, PubMed:12228231). Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation

• Expression：
• Ubiquitous.

Inhibitor GSK690693 extends Drosophila lifespan via reduce AKT signaling pathway Mech Ageing Dev. 2022 Mar;202:111633. WB Drosophila

Overexpression of HepaCAM inhibits bladder cancer cell proliferation and viability through the AKT/FoxO pathway. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY J Cancer Res Clin. 2017 May;143(5):793-805 WB Human 1:1000 Bca cell

Screening active components from Rubus amabilis for pancreatic β-cells protection. PHARMACEUTICAL BIOLOGY Pharm Biol. 2020;58(1):674-685 WB Mouse MIN6 cells

Paclitaxel alleviates monocrotaline-induced pulmonary arterial hypertension via inhibition of FoxO1-mediated autophagy. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY 2019 Jan 25 WB Rat 1:1000 lung tissues

Effects of metformin on clinical, hormonal and relevant gene expression parameters in patients of acne – an observational study CLINICAL AND EXPERIMENTAL DERMATOLOGY Dipankar De IHC Human sebaceous glands, eccrine glands keratinocytes

Nutritional Programming of the Lifespan of Male Drosophila by Activating FOXO on Larval Low-Nutrient Diet Nutrients Mingyao Yang WB Fruit fly body

The adaptor protein 14-3-3zeta modulates intestinal immunity and aging in Drosophila. JOURNAL OF BIOLOGICAL CHEMISTRY Mingyao Yang WB Flies，Human 293T cell

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