• Target：
• NFkB p65

• Fields：
• >>Antifolate resistance;>>MAPK signaling pathway;>>Ras signaling pathway;>>cAMP signaling pathway;>>Chemokine signaling pathway;>>NF-kappa B signaling pathway;>>HIF-1 signaling pathway;>>Sphingolipid signaling pathway;>>Mitophagy - animal;>>PI3K-Akt signaling pathway;>>Apoptosis;>>Longevity regulating pathway;>>Cellular senescence;>>Osteoclast differentiation;>>Neutrophil extracellular trap formation;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>RIG-I-like receptor signaling pathway;>>Cytosolic DNA-sensing pathway;>>C-type lectin receptor signaling pathway;>>IL-17 signaling pathway;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>T cell receptor signaling pathway;>>B cell receptor signaling pathway;>>TNF signaling pathway;>>Neurotrophin signaling pathway;>>Prolactin signaling pathway;>>Adipocytokine signaling pathway;>>Relaxin signaling pathway;>>Insulin resistance;>>Non-alcoholic fatty liver disease;>>AGE-RAGE signaling pathway in diabe

• Gene Name：
• RELA

• Protein Name：
• Transcription factor p65

• Human Gene Id：
• 5970

• Human Swiss Prot No：
• Q04206

• Mouse Gene Id：
• 19697

• Mouse Swiss Prot No：
• Q04207

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the phosphorylation site of Ser536. AA range:502-551

• Specificity：
• Phospho-NFκB-p65 (S536) Polyclonal Antibody detects endogenous levels of NFκB-p65 protein only when phosphorylated at S536.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. Immunoprecipitation: 2-5 ug:mg lysate. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• RELA;NFKB3;Transcription factor p65;Nuclear factor NF-kappa-B p65 subunit;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

• Observed Band(KD)：
• 60kD

• Background：
• NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],

• Function：
• function:NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by in

• Subcellular Location：
• Nucleus . Cytoplasm . Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B) (PubMed:1493333). Colocalized with DDX1 in the nucleus upon TNF-alpha induction (PubMed:19058135). Colocalizes with GFI1 in the nucleus after LPS stimulation (PubMed:20547752). Translocation to the nucleus is impaired in L.monocytogenes infection (PubMed:20855622). .

• Expression：
• Bone,Colon,Pancreas,Placenta,

Tyrosol inhibits NF-κB pathway in the treatment of enterotoxigenic Escherichia coli-induced diarrhea in mice MICROBIAL PATHOGENESIS Fazheng Yu, Jian Guo, Hong lin Ren, Shiying Lu, Zhaoqi He, Jiang Chang, Xueyu Hu, Ruoran Shi, Yuanyuan Jin, Yansong Li, Zengshan Liu, Xiaoxu Wang, Pan Hu WB Mouse colonic tissue

STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice WB Mouse 1:1000 /KCs, hepatocytes

High expression level of CXCL1/GROα is linked to advanced stage and worse survival in uterine cervical cancer and facilitates tumor cell malignant processes WB Human 1:1000 /HeLa cell

Grafting Resveratrol onto Mesoporous Silica Nanoparticles towards Efficient Sustainable Immunoregulation and Insulin Resistance Alleviation for Diabetic Periodontitis Therapy WB Mouse 1 : 500 /RAW 264.7 cell

Bacillus subtilis ameliorates Escherichia coli-induced endometritis in mice via maintaining endometrial barrier and inhibiting inflammatory response. MICROBIAL PATHOGENESIS2022 May;166:105487. Mouse uterine tissue

Stellate ganglion block reduces inflammation and improves neurological function in diabetic rats during ischemic stroke Neural Regen Res. 2022 Feb;17(9):1991. WB Rat 1:2000 cerebral cortex

Esculetin inhibits the pyroptosis of microvascular endothelial cells through NF-κB /NLRP3 signaling pathway Arch Biochem Biophys. 2022 May;720:109173. WB Rat

Overexpression of angiotensin-converting enzyme 2 contributes to the amelioration of Streptococcus uberis-induced inflammatory injury in mammary epithelial cells Vet Microbiol. 2022 May;268:109398. WB Mouse

Electroacupuncture Prevents the Depression-Like Behavior by Inhibiting the NF-κB/NLRP3 Inflammatory Pathway in Hippocampus of Mice Subjected to Chronic Mild Stress Neuropsychobiology. ;:1-9. WB Mouse 1:1000 hippocampus

Imidacloprid increases intestinal permeability by disrupting tight junctions. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Ecotox Environ Safe. 2021 Oct;222:112476 WB Rat 1:1000 Intestine

SPNS2 Downregulation Induces EMT and Promotes Colorectal Cancer Metastasis via Activating AKT Signaling Pathway. Frontiers in Oncology Front Oncol. 2021 Jun 24;11:682773 WB Human 1:1000 HCT116 cell, SW480 cells

MAST3 modulates the inflammatory response and proliferation of fibroblast-like synoviocytes in rheumatoid arthritis. INTERNATIONAL IMMUNOPHARMACOLOGY Int Immunopharmacol. 2019 Dec;77:105900 WB Rat 1:500 Synovial tissues FLSs

Enhanced autophagy reveals vulnerability of P-gp mediated epirubicin resistance in triple negative breast cancer cells. APOPTOSIS Apoptosis. 2016 Apr;21(4):473-488 WB Human MDA-MB-231 cell

Human Milk Oligosaccharides Protect against Necrotizing Enterocolitis by Inhibiting Intestinal Damage via Increasing the Proliferation of Crypt Cells. MOLECULAR NUTRITION & FOOD RESEARCH Mol Nutr Food Res. 2019 Sep;63(18):1900262 WB Mouse 1:100 distal ileum

Human milk oligosaccharides 3′-sialyllactose and 6′-sialyllactose protect intestine against necrotizing enterocolitis damage induced by hypoxia. Journal of Functional Foods J Funct Foods. 2021 Nov;86:104708 IF Human 1:100 Ileum

NLRC5 promotes cell proliferation via regulating the NF-κB signaling pathway in Rheumatoid arthritis. MOLECULAR IMMUNOLOGY Mol Immunol. 2017 Nov;91:24 WB Rat 1:500 Synovial tissues FLS cell

Synergistic suppression effect on tumor growth of acute myeloid leukemia by combining cytarabine with an engineered oncolytic vaccinia virus. OncoTargets and Therapy Oncotargets Ther. 2018; 11: 6887–6900 WB Human 1:1000 Leukemia cell

E3 ligase ASB8 promotes porcine reproductive and respiratory syndrome virus proliferation by stabilizing the viral Nsp1α protein and degrading host IKKβ kinase. VIROLOGY Virology. 2019 Jun;532:55 WB Human HEK293T cell

PSTPIP2 Inhibits the Inflammatory Response and Proliferation of Fibroblast-Like Synoviocytes in vitro. Frontiers in Pharmacology Front Pharmacol. 2018 Dec;9:1432 WB Rat 1:1000 Synovial tissues Fibroblast-like synoviocytes (FLSs)

IL-17A and IL-17F repair HIV-1 gp140 damaged Caco-2 cell barriers by upregulating tight junction genes. MICROBES AND INFECTION 2019 Apr 02 WB Human Caco-2 cell

Lactoferrin suppresses lipopolysaccharide-induced endometritis in mice via down-regulation of the NF-κB pathway. INTERNATIONAL IMMUNOPHARMACOLOGY Int Immunopharmacol. 2015 Sep;28:695 WB Mouse 1:1000 uterine tissues

Deoxycholic Acid Upregulates Serum Golgi Protein 73 through Activating NF-κB Pathway and Destroying Golgi Structure in Liver Disease. Biomolecules Biomolecules. 2021 Feb;11(2):205 WB Human Huh-7 cell,SMMC7721 cell

Ganoderic Acids Prevent Renal Ischemia Reperfusion Injury by Inhibiting Inflammation and Apoptosis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Int J Mol Sci. 2021 Jan;22(19):10229 WB Rat Renal tissues NRK-52E cell

Elevated c-fos expression is correlated with phenotypic switching of human vascular smooth muscle cells derived from lower limb venous varicosities. Journal of Vascular Surgery-Venous and Lymphatic Disorders J Vasc Surg-Venous L. 2021 Jan;9:242 WB Human 1:1000 VSMCs

Berberine hydrochloride attenuates lipopolysaccharide-induced endometritis in mice by suppressing activation of NF-κB signal pathway. INTERNATIONAL IMMUNOPHARMACOLOGY 2014 Nov 15 WB Mouse uteri

The receptor for activated protein kinase C promotes cell growth, invasion and migration in cervical cancer. INTERNATIONAL JOURNAL OF ONCOLOGY Int J Oncol. 2017 Nov;51(5):1497-1507 WB Human 1:1000 cervical cancer tissues CaSki cell

Dopamine receptor D2 inhibition alleviates diabetic hepatic stellate cells fibrosis by regulating the TGF‐β1/Smads and NFκB pathways. CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY Clin Exp Pharmacol P. 2021 Mar;48(3):370-380 WB Rat 1 : 1000 Liver hepatic stellate cells (HSCs)

The inflammation and reactive oxygen species regulated by Nrf2 and NF-κB signaling pathways in 630-nm light-emitting diode irradiation treated THP-1 monocytes/macrophages. LASERS IN MEDICAL SCIENCE 2020 Oct 31 WB Human 1:1000 THP-1 cell

Preventive effects of donkey milk powder on the ovalbumin-induced asthmatic mice. Journal of Functional Foods J Funct Foods. 2021 Sep;84:104603 IHC Mouse Lung

Huoxin Pill Attenuates Cardiac Inflammation by Suppression of TLR4/NF-κB in Acute Myocardial Ischemia Injury Rats. Evidence-based Complementary and Alternative Medicine 2020 Jul 09 IHC Rat 1 : 200 Cardiac tissue

Aβ40 Promotes the Osteoblastic Differentiation of Aortic Valve Interstitial Cells through the RAGE Pathway. Current Medical Science 2020 Oct 29 WB Human 1:500 Acute aortic aortic valve interstitial cells (AVICs)

Helal, Manar, et al. "Lactoferrin Ameliorates Azithromycin-induced Cardiac Injury: Insight into Oxidative Stress/TLR4/NF-κB Pathway." Journal of Advanced Pharmacy Research 5.2 (2021): 285-296.

Chen, Jia, et al. "Network Pharmacology-Based Strategy for Elucidating the Molecular Basis Forthe Pharmacologic Effects of Licorice (Glycyrrhiza spp.)." Frontiers in Pharmacology 12 (2021): 872.

Li, Ting-ting, et al. "Stellate Ganglion Block Brain Protection During Ischemic Stroke in Diabetes Rats: TLR4/NF-κB Pathway and Inflammation." (2020).

Induction of breast cancer stem cells by M1 macrophages through Lin-28B-let-7-HMGA2 axis. CANCER LETTERS 2019 Mar 24 WB Human MCF-7 cell

Establishment and Mechanism Study of a Primary Ovarian Insufficiency Mouse Model Using Lipopolysaccharide. Analytical Cellular Pathology Anal Cell Pathol. 2021;2021:1781532 WB Mouse 1:1000 Ovarian tissue

Molecular Hydrogen Mediates Neurorestorative Effects After Stroke in Diabetic Rats: the TLR4/NF-κB Inflammatory Pathway Journal of Neuroimmune Pharmacology Wen-zhi Li WB Rat

TcpC Inhibits M1 but Promotes M2 Macrophage Polarization via Regulation of the MAPK/NF-κB and Akt/STAT6 Pathways in Urinary Tract Infection Cells Jianping Pan WB Human,Mouse

Amelioration of Lung Fibrosis by Total Flavonoids of Astragalus via Inflammatory Modulation and Epithelium Regeneration AMERICAN JOURNAL OF CHINESE MEDICINE Min Li WB Human,Mouse lung tissues A549 cells

Protective effect of astaxanthin on tuberculosis-associated inflammatory lung injury EXPERIMENTAL BIOLOGY AND MEDICINE Xiaoguang Zou WB Mouse MLE-12 cells

Effects of Aire on perforin expression in BMDCs via TLR7/8 and its therapeutic effect on type 1 diabetes INTERNATIONAL IMMUNOPHARMACOLOGY Wei Yang WB Mouse bone marrow-derived dendritic cells (BMDCs)

Scavenger receptor A-mediated nanoparticles target M1 macrophages for acute liver injury. Tao Gong WB Mouse 1:900 liver tissue

Gentianella acuta-derived Gen-miR-1 suppresses myocardial fibrosis by targeting HAX1/HMG20A/Smads axis to attenuate inflammation in cardiac fibroblasts. Aiying Li WB Rat 1:1000 Heart tissue cardiac fibroblasts (CFs)

Manganese ion gradient liposomes modified by disulfide bond enhance cellular uptake and tumor immunity. Haiyang Hu WB Mouse B16F10 cell-xenograft,4T1 cell-xenograft

Quercitrin attenuates the progression of osteoarthritis via inhibiting NF-κB signaling pathways and enhance glucose transport capacity EXPERIMENTAL CELL RESEARCH Shigang Qiao IHC Rat 1:300 knee

Baicalein ameliorates cognitive impairment of vascular dementia rats via suppressing neuroinflammation and regulating intestinal microbiota BRAIN RESEARCH BULLETIN Jiaxi Song WB Rat 1:1000 Hippocampus

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