• Target：
• MCM4

• Fields：
• >>DNA replication;>>Cell cycle

• Gene Name：
• MCM4

• Protein Name：
• DNA replication licensing factor MCM4

• Human Gene Id：
• 4173

• Human Swiss Prot No：
• P33991

• Mouse Gene Id：
• 17217

• Mouse Swiss Prot No：
• P49717

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human MCM4 around the phosphorylation site of Ser54. AA range:20-69

• Specificity：
• Phospho-MCM4 (S54) Polyclonal Antibody detects endogenous levels of MCM4 protein only when phosphorylated at S54.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• MCM4;CDC21;DNA replication licensing factor MCM4;CDC21 homolog;P1-CDC21

• Observed Band(KD)：
• 85kD

• Background：
• The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcri

• Function：
• function:Involved in the control of DNA replication.,similarity:Belongs to the MCM family.,similarity:Contains 1 MCM domain.,

• Subcellular Location：
• Nucleus . Chromosome . Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses. .

• Expression：
• Cervix,Epithelium,Testis,

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