• Target：
• Zyxin

• Fields：
• >>Focal adhesion

• Gene Name：
• ZYX

• Protein Name：
• Zyxin

• Human Gene Id：
• 7791

• Human Swiss Prot No：
• Q15942

• Mouse Swiss Prot No：
• Q62523

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human Zyxin around the phosphorylation site of Ser142. AA range:108-157

• Specificity：
• Phospho-Zyxin (S142) Polyclonal Antibody detects endogenous levels of Zyxin protein only when phosphorylated at S142.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• ZYX;Zyxin;Zyxin-2

• Observed Band(KD)：
• 61kD

• Background：
• Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq, Jul 2008],

• Function：
• function:Adhesion plaque protein. Binds alpha-actinin and the CRP protein. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression.,similarity:Belongs to the zyxin/ajuba family.,similarity:Contains 3 LIM zinc-binding domains.,subcellular location:Associates with the actin cytoskeleton near the adhesion plaques. Enters the nucleus in the presence of HESX1.,subunit:Interacts with HPV type 6 protein E6. Does not interact significantly with E6 proteins from HPV types 11, 16, or 18. Interacts, via the Pro-rich regions, with the EVH1 domains of ENAH and VASP. Interaction with ENA/VASP family members is important for their targeting to focal adhesions and the formation of actin-rich structures.,

• Subcellular Location：
• Cytoplasm. Cytoplasm, cytoskeleton. Nucleus. Cell junction, focal adhesion. Associates with the actin cytoskeleton near the adhesion plaques. Enters the nucleus in the presence of HESX1.

• Expression：
• Cervix carcinoma,Epididymis,Epithelium,Kidney,Placenta,Platelet,Skin,Umbilical vein,Uterus,

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