DAPK3 (phospho Thr265) Polyclonal Antibody

    • Catalog No.:YP0548
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • DAPK3
      • Fields:
      • >>Autophagy - animal;>>Pathways in cancer;>>Bladder cancer
      • Gene Name:
      • DAPK3
      • Protein Name:
      • Death-associated protein kinase 3
      • Human Gene Id:
      • 1613
      • Human Swiss Prot No:
      • O43293
      • Mouse Swiss Prot No:
      • O54784
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human DAPK3 around the phosphorylation site of Thr265. AA range:241-290
      • Specificity:
      • Phospho-DAPK3 (T265) Polyclonal Antibody detects endogenous levels of DAPK3 protein only when phosphorylated at T265.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • DAPK3;ZIPK;Death-associated protein kinase 3;DAP kinase 3;DAP-like kinase;Dlk;MYPT1 kinase;Zipper-interacting protein kinase;ZIP-kinase
      • Observed Band(KD):
      • 52kD
      • Background:
      • Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis. [provided by RefSeq, Jul 2008],
      • Function:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Associates to centromeres from prophase to anaphase.,subunit:Homodimer or forms heterodimers with ATF4. Both interactions require an intact leucine zipper domain and oligomerization is required for full enzymatic activity. Also binds to DAXX and PAWR, possibly in a ternary complex which plays a role in caspase activation. Interacts with AATF and CDC5L.,
      • Subcellular Location:
      • Nucleus . Cytoplasm . Predominantly localizes to the cytoplasm but can shuttle between the nucleus and cytoplasm; cytoplasmic localization is promoted by phosphorylation at Thr-299 and involves Rho/Rock signaling. .; [Isoform 1]: Nucleus . Cytoplasm .; [Isoform 2]: Nucleus . Cytoplasm .
      • Expression:
      • Widely expressed. Isoform 1 and isoform 2 are expressed in the bladder smooth muscle.
      • Products Images
      • Immunofluorescence analysis of Hela cell. 1,DAPK3 (phospho Thr265) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). α-tubulin Monoclonal Antibody(8F11)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
      • Western Blot analysis of HuvEc cells using Phospho-DAPK3 (T265) Polyclonal Antibody
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using DAPK3 (Phospho-Thr265) Antibody
      • Immunofluorescence analysis of A549 cells, using DAPK3 (Phospho-Thr265) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from HUVEC cells, using DAPK3 (Phospho-Thr265) Antibody. The lane on the right is blocked with the phospho peptide.
      • Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).