GABAA Rβ1 (phospho Ser434) Polyclonal Antibody
- Catalog No.：YP0576
- Applications：WB;IHC;IF;ELISA
- Reactivity：Human;Mouse;Rat;Monkey
- Target：
- GABAA Rβ1
- Fields：
- >>Neuroactive ligand-receptor interaction;>>Retrograde endocannabinoid signaling;>>Serotonergic synapse;>>GABAergic synapse;>>Morphine addiction;>>Nicotine addiction
- Gene Name：
- GABRB1
- Protein Name：
- Gamma-aminobutyric acid receptor subunit beta-1
- Human Gene Id：
- 2560
- Human Swiss Prot No：
- P18505
- Mouse Gene Id：
- 14400
- Mouse Swiss Prot No：
- P50571
- Rat Gene Id：
- 25450
- Rat Swiss Prot No：
- P15431
- Immunogen：
- The antiserum was produced against synthesized peptide derived from human GABA-RB around the phosphorylation site of Ser434. AA range:401-450
- Specificity：
- Phospho-GABAA Rβ1 (S434) Polyclonal Antibody detects endogenous levels of GABAA Rβ1 protein only when phosphorylated at S434.
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Polyclonal, Rabbit,IgG
- Dilution：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
- Purification：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name：
- GABRB1;Gamma-aminobutyric acid receptor subunit beta-1;GABA(A) receptor subunit beta-1
- Observed Band(KD)：
- 55kD
- Background：
- The gamma-aminobutyric acid (GABA) A receptor is a multisubunit chloride channel that mediates the fastest inhibitory synaptic transmission in the central nervous system. This gene encodes GABA A receptor, beta 1 subunit. It is mapped to chromosome 4p12 in a cluster comprised of genes encoding alpha 4, alpha 2 and gamma 1 subunits of the GABA A receptor. Alteration of this gene is implicated in the pathogenetics of schizophrenia. [provided by RefSeq, Jul 2008],
- Function：
- function:GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.,online information:Forbidden fruit - Issue 56 of March 2005,similarity:Belongs to the ligand-gated ionic channel (TC 1.A.9) family.,subunit:Binds UBQLN1 (By similarity). Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.,
- Subcellular Location：
- Cell junction, synapse, postsynaptic cell membrane ; Multi-pass membrane protein . Cell membrane ; Multi-pass membrane protein .
- Expression：
- Brain,Epithelium,
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- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using Phospho-GABAA Rβ1 (S434) Polyclonal Antibody
- Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using GABA-RB (Phospho-Ser434) Antibody
- Immunohistochemistry analysis of paraffin-embedded human brain, using GABA-RB (Phospho-Ser434) Antibody. The picture on the right is blocked with the phospho peptide.
- Western blot analysis of lysates from COS7 cells, using GABA-RB (Phospho-Ser434) Antibody. The lane on the right is blocked with the phospho peptide.
