• Target：
• LIMK-1/2

• Fields：
• >>Axon guidance;>>Fc gamma R-mediated phagocytosis;>>Regulation of actin cytoskeleton;>>Yersinia infection;>>Human immunodeficiency virus 1 infection

• Gene Name：
• LIMK1/LIMK2

• Protein Name：
• LIM domain kinase 1/LIM domain kinase 2

• Human Gene Id：
• 3984/3985

• Human Swiss Prot No：
• P53667/P53671

• Mouse Gene Id：
• 16885/16886

• Rat Gene Id：
• 29524

• Rat Swiss Prot No：
• P53669/P53670

• Immunogen：
• Synthesized phospho-peptide around the phosphorylation site of human LIMK-1/2 (phospho Thr508/505)

• Specificity：
• Phospho-LIMK-1/2 (T508/505) Polyclonal Antibody detects endogenous levels of LIMK-1/2 protein only when phosphorylated at T508/505.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000;IHC 1:100-500;IF ICC 1:100-500;ELISA 1:5000-20000

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• LIMK1;LIMK;LIM domain kinase 1;LIMK-1;LIMK2;LIM domain kinase 2;LIMK-2

• Observed Band(KD)：
• 72kD

• Background：
• There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cog

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Haploinsufficiency of LIMK1 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23.,function:Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. May be involved in brain development.,PTM:Autophosphorylated.,PTM:Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.,similarity:Contains 1 PDZ (DHR) doma

• Subcellular Location：
• Cytoplasm . Nucleus . Cytoplasm, cytoskeleton . Cell projection, lamellipodium . Predominantly found in the cytoplasm. Localizes in the lamellipodium in a CDC42BPA, CDC42BPB and FAM89B/LRAP25-dependent manner. .

• Expression：
• Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.

LIMK1/2 in the mPFC Plays a Role in Chronic Stress-Induced Depressive-Like Effects in Mice. INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY 2020 Dec 29 IF Mouse 1:100 medial prefrontal cortex (mPFC)

LKB1 loss promotes colorectal cancer cell metastasis through regulating TNIK expression and actin cytoskeleton remodeling. Mei Liu WB Human 1:1000 HCT116 cell

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