• Target：
• FADD

• Fields：
• >>Platinum drug resistance;>>Apoptosis;>>Apoptosis - multiple species;>>Necroptosis;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>RIG-I-like receptor signaling pathway;>>IL-17 signaling pathway;>>TNF signaling pathway;>>Alcoholic liver disease;>>Alzheimer disease;>>Pathways of neurodegeneration - multiple diseases;>>Pathogenic Escherichia coli infection;>>Salmonella infection;>>Chagas disease;>>Tuberculosis;>>Hepatitis C;>>Hepatitis B;>>Measles;>>Human cytomegalovirus infection;>>Influenza A;>>Human papillomavirus infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Herpes simplex virus 1 infection;>>Epstein-Barr virus infection;>>Human immunodeficiency virus 1 infection;>>Pathways in cancer

• Gene Name：
• FADD

• Protein Name：
• Protein FADD

• Human Gene Id：
• 8772

• Human Swiss Prot No：
• Q13158

• Mouse Swiss Prot No：
• Q61160

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human FADD around the phosphorylation site of Ser194. AA range:159-208

• Specificity：
• Phospho-FADD (S194) Polyclonal Antibody detects endogenous levels of FADD protein only when phosphorylated at S194.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• FADD;MORT1;GIG3;Protein FADD;FAS-associated death domain protein;FAS-associating death domain-containing protein;Growth-inhibiting gene 3 protein;Mediator of receptor induced toxicity

• Observed Band(KD)：
• 28kD

• Background：
• The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq, Jul 2008],

• Function：
• domain:Contains a death domain involved in the binding of the corresponding domain within Fas receptor.,function:Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.,PTM:Phosphorylated.,similarity:Contains 1 death domain.,similarity:Contains 1 DED (death effector) domain.,subunit:Interacts with CFLAR, PEA15 and MBD4. When phosphorylated, part of a complex containing HIPK3 and FAS. May interact with MAVS/IPS1. Interacts with MOCV v-CFLAR protein and LRDD.,tissue specificity:Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.,

• Subcellular Location：
• cytoplasm,cytosol,plasma membrane,death-inducing signaling complex,CD95 death-inducing signaling complex,neuron projection,cell body,membrane raft,ripoptosome,

• Expression：
• Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs