IL-3Rβ (phospho Tyr593) Polyclonal Antibody
- Catalog No.:YP0639
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- IL-3Rβ
- Fields:
- >>Cytokine-cytokine receptor interaction;>>Apoptosis;>>JAK-STAT signaling pathway;>>Pathways in cancer
- Gene Name:
- CSF2RB
- Protein Name:
- Cytokine receptor common subunit beta
- Human Gene Id:
- 1439
- Human Swiss Prot No:
- P32927
- Mouse Gene Id:
- 12983
- Mouse Swiss Prot No:
- P26955
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human IL-3R beta around the phosphorylation site of Tyr593. AA range:559-608
- Specificity:
- Phospho-IL-3Rβ (Y593) Polyclonal Antibody detects endogenous levels of IL-3Rβ protein only when phosphorylated at Y593.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- CSF2RB;IL3RB;IL5RB;Cytokine receptor common subunit beta;CDw131;GM-CSF/IL-3/IL-5 receptor common beta subunit;CD antigen CD131
- Observed Band(KD):
- 125kD
- Background:
- The protein encoded by this gene is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this gene have been reported to be associated with protein alveolar proteinosis (PAP). [provided by RefSeq, Jul 2008],
- Function:
- disease:Defects in CSF2RB are a cause of congenital pulmonary alveolar proteinosis (PAP) [MIM:265120]. PAP is an autosomal recessive fatal respiratory disease.,domain:The box 1 motif is required for JAK interaction and/or activation.,domain:The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.,function:High affinity receptor for interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor.,similarity:Belongs to the type I cytokine receptor family. Type 4 subfamily.,similarity:Contains 2 fibronectin type-III domains.,subunit:Heterodimer of an alpha and a beta subunit. The beta subunit is common to the IL3, IL5 and GM-CSF receptors.,
- Subcellular Location:
- Membrane; Single-pass type I membrane protein.
- Expression:
- Placenta,
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- Antibody-FAQs
- Products Images
- Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IL-3R beta (Phospho-Tyr593) Antibody
- Immunohistochemistry analysis of paraffin-embedded human brain, using IL-3R beta (Phospho-Tyr593) Antibody. The picture on the right is blocked with the phospho peptide.
- Western blot analysis of lysates from RAW264.7 cells treated with G-CSF 25ng/ml 15', using IL-3R beta (Phospho-Tyr593) Antibody. The lane on the right is blocked with the phospho peptide.