• Target：
• FER

• Fields：
• >>Adherens junction

• Gene Name：
• FER

• Protein Name：
• Tyrosine-protein kinase Fer

• Human Gene Id：
• 2241

• Human Swiss Prot No：
• P16591

• Mouse Gene Id：
• 14158

• Mouse Swiss Prot No：
• P70451

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human FER around the phosphorylation site of Tyr402. AA range:371-420

• Specificity：
• Phospho-Fer (Y402) Polyclonal Antibody detects endogenous levels of Fer protein only when phosphorylated at Y402.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• FER;TYK3;Tyrosine-protein kinase Fer;Feline encephalitis virus-related kinase FER;Fujinami poultry sarcoma/Feline sarcoma-related protein Fer;Proto-oncogene c-Fer;Tyrosine kinase 3;p94-Fer

• Observed Band(KD)：
• 85kD

• Background：
• The protein encoded by this gene is a member of the FPS/FES family of non-transmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome X. [provided by RefSeq, Apr 2015],

• Function：
• catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Tyrosine kinase of the non-receptor type. Probably performs an important function, perhaps in regulatory processes such as cell cycle control.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.,similarity:Contains 1 FCH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,subcellular location:Associated with the chromatin.,tissue specificity:Expressed in a variety of lymphoid cell lines.,

• Subcellular Location：
• Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection. Cell junction. Membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus. Cytoplasm, cell cortex. Associated with the chromatin. Detected on microtubules in polarized and motile vascular endothelial cells. Colocalizes with F-actin at the cell cortex. Colocalizes with PECAM1 and CTNND1 at nascent cell-cell contacts.

• Expression：
• Isoform 1 is detected in normal colon and in fibroblasts (at protein level). Isoform 3 is detected in normal testis, in colon carcinoma-derived metastases in lung, liver and ovary, and in colon carcinoma and hepato carcinoma cell lines (at protein level). Isoform 3 is not detected in normal colon or in normal fibroblasts (at protein level). Widely expressed.

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