MEK Kinase-1 (phospho Thr1402) Polyclonal Antibody
- Catalog No.：YP0786
- Applications：WB;IHC;IF;ELISA
- Reactivity：Human;Mouse;Rat
- Target：
- MEK Kinase-1
- Fields：
- >>MAPK signaling pathway;>>Ubiquitin mediated proteolysis;>>Tight junction;>>RIG-I-like receptor signaling pathway;>>Neurotrophin signaling pathway;>>GnRH signaling pathway;>>Growth hormone synthesis, secretion and action;>>Hepatitis B;>>Human T-cell leukemia virus 1 infection
- Gene Name：
- MAP3K1
- Protein Name：
- Mitogen-activated protein kinase kinase kinase 1
- Human Gene Id：
- 4214
- Human Swiss Prot No：
- Q13233
- Mouse Swiss Prot No：
- P53349
- Rat Gene Id：
- 116667
- Rat Swiss Prot No：
- Q62925
- Immunogen：
- The antiserum was produced against synthesized peptide derived from human MAP3K1 around the phosphorylation site of Thr1402. AA range:1368-1417
- Specificity：
- Phospho-MEK Kinase-1 (T1402) Polyclonal Antibody detects endogenous levels of MEK Kinase-1 protein only when phosphorylated at T1402.
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Polyclonal, Rabbit,IgG
- Dilution：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
- Purification：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name：
- MAP3K1;MAPKKK1;MEKK;MEKK1;Mitogen-activated protein kinase kinase kinase 1;MAPK/ERK kinase kinase 1;MEK kinase 1;MEKK 1
- Observed Band(KD)：
- 160kD
- Background：
- The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus. [provided by RefSeq, Mar 2012],
- Function：
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by autophosphorylation on Thr-1400 and Thr-1412 following oligomerization.,function:Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 1 SWIM-type zinc finger.,subunit:Binds both upstream activators and downstream substrates in multimolecular complexes through its N-terminus. Oligomerizes after binding MAP4K2 or TRAF2. Interacts with AXIN1.,
- Subcellular Location：
- cytoplasm,cytosol,
- Expression：
- Leukocyte,
- June 19-2018
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- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
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- July 13-2018
- Antibody-FAQs
- Products Images
- Western Blot analysis of NIH-3T3 cells using Phospho-MEK Kinase-1 (T1402) Polyclonal Antibody diluted at 1:2000
- Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MAP3K1 (Phospho-Thr1402) Antibody
- Immunohistochemistry analysis of paraffin-embedded human brain, using MAP3K1 (Phospho-Thr1402) Antibody. The picture on the right is blocked with the phospho peptide.
- Western blot analysis of lysates from Jurkat cells, using MAP3K1 (Phospho-Thr1402) Antibody. The lane on the right is blocked with the phospho peptide.
