Ephrin-B1/2/3 (phospho Tyr324) Polyclonal Antibody
- Catalog No.：YP0812
- Applications：WB;IHC;IF;ELISA
- Reactivity：Human;Mouse;Rat
- Target：
- Ephrin-B1/2/3
- Fields：
- >>Axon guidance
- Gene Name：
- EFNB1/EFNB2/EFNB3
- Protein Name：
- Ephrin-B1/2/3
- Human Gene Id：
- 1947/1948/1949
- Human Swiss Prot No：
- P98172/P52799/Q15768
- Mouse Gene Id：
- 13641/13642/13643
- Rat Swiss Prot No：
- P52796
- Immunogen：
- The antiserum was produced against synthesized peptide derived from human Ephrin B1/B2/B3 around the phosphorylation site of Tyr324. AA range:290-339
- Specificity：
- Phospho-Ephrin-B1/2/3 (Y324) Polyclonal Antibody detects endogenous levels of Ephrin-B1/2/3 protein only when phosphorylated at Y324.
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Polyclonal, Rabbit,IgG
- Dilution：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- Purification：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name：
- EFNB1;EFL3;EPLG2;LERK2;Ephrin-B1;EFL-3;ELK ligand;ELK-L;EPH-related receptor tyrosine kinase ligand 2;LERK-2;EFNB2;EPLG5;HTKL;LERK5;Ephrin-B2;EPH-related receptor tyrosine kinase ligand 5;LERK-5;HTK ligand;HTK-L;EFNB3;EP
- Observed Band(KD)：
- 46kD
- Background：
- The protein encoded by this gene is a type I membrane protein and a ligand of Eph-related receptor tyrosine kinases. It may play a role in cell adhesion and function in the development or maintenance of the nervous system. [provided by RefSeq, Jul 2008],
- Function：
- disease:Defects in EFNB1 are a cause of craniofrontonasal syndrome (CFNS) [MIM:304110]; also known as craniofrontonasal dysplasia (CFND). CFNS is an X-linked inherited syndrome characterized by hypertelorism, coronal synostosis with brachycephaly, downslanting palpebral fissures, clefting of the nasal tip, joint anomalies, longitudinally grooved fingernails and other digital anomalies.,function:Binds to the receptor tyrosine kinases EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons.,induction:By TNF-alpha.,PTM:Inducible phosphorylation of tyrosine residues in the cytoplasmic domain.,similarity:Belongs to the ephrin family.,subunit:Interacts with GRIP1 and GRIP2.,tissue specificity:Heart, placenta, lung, liver, skeletal muscle, kidney, pancreas.,
- Subcellular Location：
- Cell membrane ; Single-pass type I membrane protein . Membrane raft . May recruit GRIP1 and GRIP2 to membrane raft domains. .; [Ephrin-B1 C-terminal fragment]: Cell membrane ; Single-pass type I membrane protein .; [Ephrin-B1 intracellular domain]: Nucleus . Colocalizes with ZHX2 in the nucleus. .
- Expression：
- Widely expressed (PubMed:8070404, PubMed:7973638). Detected in both neuronal and non-neuronal tissues (PubMed:8070404, PubMed:7973638). Seems to have particularly strong expression in retina, sciatic nerve, heart and spinal cord (PubMed:7973638).
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- Antibody-FAQs
- Products Images
- Western Blot analysis of JK cells using Phospho-Ephrin-B1/2/3 (Y324) Polyclonal Antibody
- Immunohistochemistry analysis of paraffin-embedded human brain, using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody. The picture on the right is blocked with the phospho peptide.
- Western blot analysis of lysates from K562 cells treated with serum 20% 15', using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody. The lane on the right is blocked with the phospho peptide.
