• Target：
• Mnk1

• Fields：
• >>MAPK signaling pathway;>>HIF-1 signaling pathway;>>Insulin signaling pathway

• Gene Name：
• MKNK1

• Protein Name：
• MAP kinase-interacting serine/threonine-protein kinase 1

• Human Gene Id：
• 8569

• Human Swiss Prot No：
• Q9BUB5

• Mouse Gene Id：
• 17346

• Mouse Swiss Prot No：
• O08605

• Rat Gene Id：
• 500526

• Rat Swiss Prot No：
• Q4G050

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human Mnk1 around the phosphorylation site of Thr385. AA range:351-400

• Specificity：
• Phospho-Mnk1 (T385) Polyclonal Antibody detects endogenous levels of Mnk1 protein only when phosphorylated at T385.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• MKNK1;MNK1;MAP kinase-interacting serine/threonine-protein kinase 1;MAP kinase signal-integrating kinase 1;MAPK signal-integrating kinase 1;Mnk1

• Observed Band(KD)：
• 42kD

• Background：
• MAP kinase interacting serine/threonine kinase 1(MKNK1) Homo sapiens This gene encodes a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. This kinase may also regulate transcription by phosphorylating eIF4E via interaction with the C-terminal region of eIF4G. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Jan 2012],

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Phosphorylated and activated by the p38 kinases and kinases in the Erk pathway.,function:May play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.,PTM:Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with the C-terminal regions of EIF4G1 and EIF4G2. Also binds to dephosphorylated ERK1 and ERK2, and to the p38 kinases.,tissue specificity:Ubiquitous.,

• Subcellular Location：
• [Isoform 2]: Cytoplasm.; [Isoform 3]: Cytoplasm. Nucleus.

• Expression：
• Ubiquitous.

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