IRS-1 (phospho Ser323) Polyclonal Antibody

    • Catalog No.：YP0881
    • Applications：WB;IHC;IF;ELISA
    • Reactivity：Human;Mouse;Rat;Monkey
      • Target：
      • IRS-1
      • Fields：
      • >>cGMP-PKG signaling pathway;>>FoxO signaling pathway;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Neurotrophin signaling pathway;>>Insulin signaling pathway;>>Adipocytokine signaling pathway;>>Regulation of lipolysis in adipocytes;>>Type II diabetes mellitus;>>Insulin resistance;>>Non-alcoholic fatty liver disease;>>Growth hormone synthesis, secretion and action;>>Aldosterone-regulated sodium reabsorption;>>Alzheimer disease;>>MicroRNAs in cancer;>>Diabetic cardiomyopathy
      • Gene Name：
      • IRS1
      • Protein Name：
      • Insulin receptor substrate 1
      • Human Gene Id：
      • 3667
      • Human Swiss Prot No：
      • P35568
      • Mouse Swiss Prot No：
      • P35569
      • Immunogen：
      • The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Ser323. AA range:289-338
      • Specificity：
      • Phospho-IRS-1 (S323) Polyclonal Antibody detects endogenous levels of IRS-1 protein only when phosphorylated at S323.
      • Formulation：
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source：
      • Polyclonal, Rabbit,IgG
      • Dilution：
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
      • Purification：
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration：
      • 1 mg/ml
      • Storage Stability：
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name：
      • IRS1;Insulin receptor substrate 1;IRS-1
      • Observed Band(KD)：
      • 170kD
      • Background：
      • This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009],
      • Function：
      • disease:Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].,function:May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.,polymorphism:The Arg-971 polymorphism impairs the ability of insulin to stimulate glucose transport, glucose transporter translocation, and glycogen synthesis by affecting the PI3K/AKT1/GSK3 signaling pathway. The polymorphism at Arg-971 may contribute to the in vivo insulin resistance observed in carriers of this variant. Arg-971 could contribute to the risk for atherosclerotic cardiovascular diseases associated with non-insulin-dependen
      • Subcellular Location：
      • nucleus,cytoplasm,cytosol,plasma membrane,insulin receptor complex,caveola,intracellular membrane-bounded organelle,
      • Expression：
      • Epithelium,Eye,Skeletal muscle,
      • Products Images
      • Western blot analysis of 293T using p-IRS-1 (S323) antibody. Antibody was diluted at 1:1000
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IRS-1 (Phospho-Ser323) Antibody
      • Immunofluorescence analysis of HeLa cells, using IRS-1 (Phospho-Ser323) Antibody. The picture on the right is blocked with the phospho peptide.
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IRS-1 (Phospho-Ser323) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from COS7 cells treated with Serum 20% 15', using IRS-1 (Phospho-Ser323) Antibody. The lane on the right is blocked with the phospho peptide.

      Attributes

      • Code：
      • YP0881
      • Size
      • 50μL / 100μL / 200μL
      • Price$：
      • 190 / 340 / 600
      • Delivery：
      • 2024-05-07
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