• Target：
• A-Raf

• Fields：
• >>EGFR tyrosine kinase inhibitor resistance;>>Endocrine resistance;>>MAPK signaling pathway;>>ErbB signaling pathway;>>FoxO signaling pathway;>>Vascular smooth muscle contraction;>>Natural killer cell mediated cytotoxicity;>>Long-term potentiation;>>Serotonergic synapse;>>Long-term depression;>>Regulation of actin cytoskeleton;>>Insulin signaling pathway;>>Progesterone-mediated oocyte maturation;>>Parathyroid hormone synthesis, secretion and action;>>Alzheimer disease;>>Pathways of neurodegeneration - multiple diseases;>>Alcoholism;>>Hepatitis C;>>Hepatitis B;>>Pathways in cancer;>>Proteoglycans in cancer;>>Chemical carcinogenesis - reactive oxygen species;>>Colorectal cancer;>>Renal cell carcinoma;>>Pancreatic cancer;>>Endometrial cancer;>>Glioma;>>Prostate cancer;>>Melanoma;>>Bladder cancer;>>Chronic myeloid leukemia;>>Acute myeloid leukemia;>>Non-small cell lung cancer;>>Breast cancer;>>Hepatocellular carcinoma;>>Gastric cancer

• Gene Name：
• ARAF

• Protein Name：
• Serine/threonine-protein kinase A-Raf

• Human Gene Id：
• 369

• Human Swiss Prot No：
• P10398

• Mouse Gene Id：
• 11836

• Mouse Swiss Prot No：
• P04627

• Rat Gene Id：
• 64363

• Rat Swiss Prot No：
• P14056

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human A-RAF around the phosphorylation site of Tyr302. AA range:276-325

• Specificity：
• Phospho-A-Raf (Y302) Polyclonal Antibody detects endogenous levels of A-Raf protein only when phosphorylated at Y302.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• ARAF;ARAF1;PKS;PKS2;Serine/threonine-protein kinase A-Raf;Proto-oncogene A-Raf;Proto-oncogene A-Raf-1;Proto-oncogene Pks

• Observed Band(KD)：
• 68kD

• Background：
• This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012],

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Binds 2 zinc ions per subunit.,function:Involved in the transduction of mitogenic signals from the cell membrane to the nucleus.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.,similarity:Contains 1 phorbol-ester/DAG-type zinc finger.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 RBD (Ras-binding) domain.,subunit:Interacts with TH1L/NELFD.,tissue specificity:Predominantly in urogenital tissues.,

• Subcellular Location：
• intracellular,mitochondrion,cytosol,

• Expression：
• Predominantly in urogenital tissues.

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