• Target：
• CaMKIα

• Fields：
• >>Calcium signaling pathway;>>Oxytocin signaling pathway;>>Aldosterone synthesis and secretion;>>Glioma

• Gene Name：
• CAMK1

• Protein Name：
• Calcium/calmodulin-dependent protein kinase type 1

• Human Gene Id：
• 8536

• Human Swiss Prot No：
• Q14012

• Mouse Gene Id：
• 52163

• Mouse Swiss Prot No：
• Q91YS8

• Rat Gene Id：
• 171503

• Rat Swiss Prot No：
• Q63450

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human CaMK1-alpha around the phosphorylation site of Thr177. AA range:143-192

• Specificity：
• Phospho-CaMKIα (T177) Polyclonal Antibody detects endogenous levels of CaMKIα protein only when phosphorylated at T177.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• CAMK1;Calcium/calmodulin-dependent protein kinase type 1;CaM kinase I;CaM-KI;CaM kinase I alpha;CaMKI-alpha

• Observed Band(KD)：
• 41kD

• Background：
• Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.,enzyme regulation:Activated by Ca(2+)/calmodulin. Binding of calmodulin results in a conformational change that generates functional binding sites for both, substrate and ATP, and thus releaves intrasteric autoinhibition. Must be phosphorylated to be maximally active. Phosphorylated by CAMKK1 or CAMKK2.,function:Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes like transcriptional regulation, hormone production, translational regulation, regulation of actin filament organization and neurite outgrowth. Involved in calcium-dependent activation of the ERK pathway (By si

• Subcellular Location：
• Cytoplasm . Nucleus . Predominantly cytoplasmic. .

• Expression：
• Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex.

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