Moesin/Ezrin/Radixin (phospho Thr558) Polyclonal Antibody
- Catalog No.：YP0940
- Applications：WB;IHC;IF;ELISA
- Reactivity：Human;Mouse;Rat
- Target：
- Moesin/Ezrin/Radixin
- Fields：
- >>Tight junction;>>Leukocyte transendothelial migration;>>Regulation of actin cytoskeleton;>>Measles;>>Proteoglycans in cancer
- Gene Name：
- MSN
- Protein Name：
- Moesin
- Human Gene Id：
- 4478/5962
- Human Swiss Prot No：
- P26038/P35241/P15311
- Mouse Gene Id：
- 17698/19684/22350
- Rat Gene Id：
- 81521/54319
- Rat Swiss Prot No：
- O35763/P31977
- Immunogen：
- The antiserum was produced against synthesized peptide derived from human Moesin/Ezrin/Radixin around the phosphorylation site of Thr558. AA range:524-573
- Specificity：
- Phospho-Moesin/Ezrin/Radixin (T558) Polyclonal Antibody detects endogenous levels of Moesin/Ezrin/Radixin protein only when phosphorylated at T558.
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Polyclonal, Rabbit,IgG
- Dilution：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
- Purification：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name：
- MSN;Moesin;Membrane-organizing extension spike protein;RDX;Radixin;EZR;VIL2;Ezrin;Cytovillin;Villin-2;p81
- Observed Band(KD)：
- 67kD
- Background：
- Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. [provided by RefSeq, Jul 2008],
- Function：
- function:Probably involved in connections of major cytoskeletal structures to the plasma membrane.,PTM:Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.,similarity:Contains 1 FERM domain.,subcellular location:Phosphorylated form is enriched in microvilli-like structures at apical membrane.,subunit:In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation (By similarity). Binds SLC9A3R1.,tissue specificity:In all tissues and cultured cells studied.,
- Subcellular Location：
- Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytoskeleton . Apical cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, microvillus membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, microvillus . Phosphorylated form is enriched in microvilli-like structures at apical membrane. Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment (By similarity). Localizes at the uropods of T lymphoblasts. .
- Expression：
- In all tissues and cultured cells studied.
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- May 20-2022
- Cell-Based ELISA│解您多样本WB检测之困扰
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Immunofluorescence analysis of A549 cells, using Moesin/Ezrin/Radixin (Phospho-Thr558) Antibody. The picture on the right is blocked with the phospho peptide.
- Immunohistochemistry analysis of paraffin-embedded human pancreas, using Moesin/Ezrin/Radixin (Phospho-Thr558) Antibody. The picture on the right is blocked with the phospho peptide.
- Western blot analysis of lysates from NIH/3T3 cells, using Moesin/Ezrin/Radixin (Phospho-Thr558) Antibody. The lane on the right is blocked with the phospho peptide.
