• Target：
• p47-phox

• Fields：
• >>Chemokine signaling pathway;>>Phagosome;>>Osteoclast differentiation;>>Neutrophil extracellular trap formation;>>Fc gamma R-mediated phagocytosis;>>Leukocyte transendothelial migration;>>Prion disease;>>Leishmaniasis;>>Chemical carcinogenesis - reactive oxygen species;>>Diabetic cardiomyopathy;>>Lipid and atherosclerosis;>>Fluid shear stress and atherosclerosis

• Gene Name：
• NCF1

• Protein Name：
• Neutrophil cytosol factor 1

• Human Gene Id：
• 653361

• Human Swiss Prot No：
• P14598

• Mouse Gene Id：
• 17969

• Mouse Swiss Prot No：
• Q09014

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human Neutrophil Cytosol Factor 1 around the phosphorylation site of Ser328. AA range:301-350

• Specificity：
• Phospho-p47-phox (S328) Polyclonal Antibody detects endogenous levels of p47-phox protein only when phosphorylated at S328.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000 IHC 1:100 - 1:300. ELISA: 1:10000. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• NCF1;NOXO2;SH3PXD1A;Neutrophil cytosol factor 1;NCF-1;47 kDa autosomal chronic granulomatous disease protein;47 kDa neutrophil oxidase factor;NCF-47K;Neutrophil NADPH oxidase factor 1;Nox organizer 2;Nox-organizing protein 2;SH3

• Molecular Weight(Da)：
• 45kD

• Background：
• The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008],

• Function：
• disease:Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.,function:NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).,online information:NCF1 deficiency database,similarity:Contains 1 PX (phox homology) domain.,similarity:Contains 2 SH3 domains.,subunit:Interacts with NOXA1.,

• Subcellular Location：
• Cytoplasm, cytosol . Membrane ; Peripheral membrane protein ; Cytoplasmic side .

• Expression：
• Detected in peripheral blood monocytes and neutrophils (at protein level).

Free fatty acids promote degranulation of azurophil granules in neutrophils by inducing production of NADPH oxidase-derived reactive oxygen species in cows with subclinical ketosis J Dairy Sci. 2022 Jan;:. WB Cow 1:1000

β-Hydroxybutyrate impairs the release of bovine neutrophil extracellular traps through inhibiting phosphoinositide 3-kinase–mediated nicotinamide adenine dinucleotide phosphate oxidase reactive oxygen species production J Dairy Sci. 2022 Feb;:. WB Bovine 1:1000

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