• Target：
• Nur77

• Fields：
• >>MAPK signaling pathway;>>PI3K-Akt signaling pathway;>>Aldosterone synthesis and secretion;>>Cortisol synthesis and secretion;>>Cushing syndrome

• Gene Name：
• NR4A1

• Protein Name：
• Nuclear receptor subfamily 4 group A member 1

• Human Gene Id：
• 3164

• Human Swiss Prot No：
• P22736

• Mouse Gene Id：
• 15370

• Mouse Swiss Prot No：
• P12813

• Rat Gene Id：
• 79240

• Rat Swiss Prot No：
• P22829

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human Nuclear Receptor NR4A1 around the phosphorylation site of Ser351. AA range:317-366

• Specificity：
• Phospho-Nur77 (S351) Polyclonal Antibody detects endogenous levels of Nur77 protein only when phosphorylated at S351.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000 IHC 1:100 - 1:300. ELISA: 1:40000. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• NR4A1;GFRP1;HMR;NAK1;Nuclear receptor subfamily 4 group A member 1;Early response protein NAK1;Nuclear hormone receptor NUR/77;Nur77;Orphan nuclear receptor HMR;Orphan nuclear receptor TR3;ST-59;Testicular receptor 3

• Observed Band(KD)：
• 65-70kD

• Background：
• This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011],

• Function：
• function:Orphan nuclear receptor.,induction:By growth-stimulating agents.,similarity:Belongs to the nuclear hormone receptor family.,similarity:Belongs to the nuclear hormone receptor family. NR4 subfamily.,similarity:Contains 1 nuclear receptor DNA-binding domain.,subunit:Interacts with GADD45GIP1.,tissue specificity:Fetal muscle and adult liver, brain and thyroid.,

• Subcellular Location：
• Nucleus . Cytoplasm . Mitochondrion . Nuclear export to the cytoplasm is XPO1-mediated and positively regulated by IFI27 (PubMed:22427340). Translocation to the mitochondrion upon interaction with RXRA and upon the presence of 9-cis retinoic acid (PubMed:17761950). .

• Expression：
• Fetal muscle and adult liver, brain and thyroid.

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