LIMK-2 (phospho Ser283) Polyclonal Antibody

    • Catalog No.：YP1028
    • Applications：WB;IHC;IF;ELISA
    • Reactivity：Human;Mouse;Rat;Monkey
      • Target：
      • LIMK-2
      • Fields：
      • >>Axon guidance;>>Fc gamma R-mediated phagocytosis;>>Regulation of actin cytoskeleton;>>Human immunodeficiency virus 1 infection
      • Gene Name：
      • LIMK2
      • Protein Name：
      • LIM domain kinase 2
      • Human Gene Id：
      • 3985
      • Human Swiss Prot No：
      • P53671
      • Mouse Swiss Prot No：
      • O54785
      • Immunogen：
      • The antiserum was produced against synthesized peptide derived from human LIMK2 around the phosphorylation site of Ser283. AA range:249-298
      • Specificity：
      • Phospho-LIMK-2 (S283) Polyclonal Antibody detects endogenous levels of LIMK-2 protein only when phosphorylated at S283.
      • Formulation：
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source：
      • Polyclonal, Rabbit,IgG
      • Dilution：
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
      • Purification：
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration：
      • 1 mg/ml
      • Storage Stability：
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name：
      • LIMK2;LIM domain kinase 2;LIMK-2
      • Observed Band(KD)：
      • 72kD
      • Background：
      • There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
      • Function：
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.,PTM:Phosphorylated on serine and/or threonine residues by ROCK1.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 LIM zinc-binding domains.,subcellular location:Isoform LIMK2a is distributed in the cytoplasm and the nucleus.,subcellular location:Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.,subunit:Binds ROCK1 and LKAP. Interacts with PARD3. Interacts with NISCH.,tissue specificity:Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, col
      • Subcellular Location：
      • Cytoplasm, cytoskeleton, spindle . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome .; [Isoform LIMK2a]: Cytoplasm . Nucleus .; [Isoform LIMK2b]: Cytoplasm . Cytoplasm, perinuclear region . Nucleus . Mainly present in the cytoplasm and is scarcely translocated to the nucleus. .
      • Expression：
      • Hepatoma,Lung,Ovary,
      • Products Images
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using LIMK2 (Phospho-Ser283) Antibody
      • Immunohistochemistry analysis of paraffin-embedded human brain, using LIMK2 (Phospho-Ser283) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of LIMK2 (Phospho-Ser283) Antibody. The lane on the right is blocked with the LIMK2 (Phospho-Ser283) peptide.

      Attributes

      • Code：
      • YP1028
      • Size
      • 50μL / 100μL / 200μL
      • Price$：
      • 190 / 340 / 600
      • Delivery：
      • 2024-12-23
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