• Target：
• IRAK-1

• Fields：
• >>MAPK signaling pathway;>>NF-kappa B signaling pathway;>>Toll-like receptor signaling pathway;>>Neurotrophin signaling pathway;>>Alcoholic liver disease;>>Pathogenic Escherichia coli infection;>>Salmonella infection;>>Pertussis;>>Yersinia infection;>>Leishmaniasis;>>Chagas disease;>>Toxoplasmosis;>>Tuberculosis;>>Hepatitis B;>>Measles;>>Herpes simplex virus 1 infection;>>Epstein-Barr virus infection;>>Human immunodeficiency virus 1 infection;>>Coronavirus disease - COVID-19;>>Lipid and atherosclerosis

• Gene Name：
• IRAK1

• Protein Name：
• Interleukin-1 receptor-associated kinase 1

• Human Gene Id：
• 3654

• Human Swiss Prot No：
• P51617

• Mouse Swiss Prot No：
• Q62406

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human IRAK1 around the phosphorylation site of Ser376. AA range:342-391

• Specificity：
• Phospho-IRAK-1 (S376) Polyclonal Antibody detects endogenous levels of IRAK-1 protein only when phosphorylated at S376.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• IRAK1;IRAK;Interleukin-1 receptor-associated kinase 1;IRAK-1

• Molecular Weight(Da)：
• 77kD

• Background：
• This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.,PTM:Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.,similarity:

• Subcellular Location：
• Cytoplasm . Nucleus . Lipid droplet . Translocates to the nucleus when sumoylated. RSAD2/viperin recruits it to the lipid droplet (By similarity). .

• Expression：
• Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.

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