• Target：
• E2A

• Fields：
• >>Signaling pathways regulating pluripotency of stem cells;>>Human T-cell leukemia virus 1 infection;>>Transcriptional misregulation in cancer

• Gene Name：
• TCF3

• Protein Name：
• Transcription factor E2-alpha

• Human Gene Id：
• 6929

• Human Swiss Prot No：
• P15923

• Mouse Gene Id：
• 21423

• Mouse Swiss Prot No：
• P15806

• Rat Gene Id：
• 171046

• Rat Swiss Prot No：
• P21677

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human E2A around the phosphorylation site of Thr355. AA range:321-370

• Specificity：
• Phospho-E2A (T355) Polyclonal Antibody detects endogenous levels of E2A protein only when phosphorylated at T355.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• TCF3;BHLHB21;E2A;ITF1;Transcription factor E2-alpha;Class B basic helix-loop-helix protein 21;bHLHb21;Immunoglobulin enhancer-binding factor E12/E47;Immunoglobulin transcription factor 1;Kappa-E2-binding factor;Transcription facto

• Molecular Weight(Da)：
• 68kD

• Background：
• This gene encodes a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may play a role in lymphoid malignancies. This gene is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1;19), with PBX1), childhood leukemia (t(19;19), with TFPT) and acute leukemia (t(12;19), with ZNF384). Alternatively spliced transcript variants encoding multiple isoforms have bee

• Function：
• disease:Chromosomal aberrations involving TCF3 are cause of forms of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with PBX1; Translocation t(17;19)(q22;p13.3) with HLF. Inversion inv(19)(p13;q13) with TFPT.,function:Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.,PTM:Phosphorylated following NGF stimulation.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with ASH1 and TWIST2. Isoform E12 interacts with GRIPE and FIGLA (By similarity). Interacts with PTF1A and TGFB1I1.

• Subcellular Location：
• Nucleus .

• Expression：
• Lymphoma,Muscle,PCR rescued clones,

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