• Target：
• EDG-1

• Fields：
• >>FoxO signaling pathway;>>Sphingolipid signaling pathway;>>Neuroactive ligand-receptor interaction

• Gene Name：
• S1PR1

• Protein Name：
• Sphingosine 1-phosphate receptor 1

• Human Gene Id：
• 1901

• Human Swiss Prot No：
• P21453

• Mouse Gene Id：
• 13609

• Mouse Swiss Prot No：
• O08530

• Rat Gene Id：
• 29733

• Rat Swiss Prot No：
• P48303

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human S1P Receptor EDG1 around the phosphorylation site of Thr236. AA range:206-255

• Specificity：
• Phospho-EDG-1 (T236) Polyclonal Antibody detects endogenous levels of EDG-1 protein only when phosphorylated at T236.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000 IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• S1PR1;CHEDG1;EDG1;Sphingosine 1-phosphate receptor 1;S1P receptor 1;S1P1;Endothelial differentiation G-protein coupled receptor 1;Sphingosine 1-phosphate receptor Edg-1;S1P receptor Edg-1;CD antigen CD363

• Observed Band(KD)：
• 42kD

• Background：
• The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016],

• Function：
• function:Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins.,induction:By the tumor promoter phorbol 12-myristate 13-acetate (PME) in the presence of cycloheximide.,PTM:S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue.,similarity:Belongs to the G-protein coupled receptor 1 family.,tissue specificity:Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.,

• Subcellular Location：
• Cell membrane ; Multi-pass membrane protein. Endosome. Membrane raft. Recruited to caveolin-enriched plasma membrane microdomains in response to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine. Ligand binding leads to receptor internalization.

• Expression：
• Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.

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