• Target：
• ERK 1/2

• Fields：
• >>EGFR tyrosine kinase inhibitor resistance;>>Endocrine resistance;>>Platinum drug resistance;>>MAPK signaling pathway;>>ErbB signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>cGMP-PKG signaling pathway;>>cAMP signaling pathway;>>Chemokine signaling pathway;>>HIF-1 signaling pathway;>>FoxO signaling pathway;>>Sphingolipid signaling pathway;>>Phospholipase D signaling pathway;>>Oocyte meiosis;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>Apoptosis;>>Cellular senescence;>>Adrenergic signaling in cardiomyocytes;>>Vascular smooth muscle contraction;>>TGF-beta signaling pathway;>>Axon guidance;>>VEGF signaling pathway;>>Apelin signaling pathway;>>Osteoclast differentiation;>>Focal adhesion;>>Adherens junction;>>Gap junction;>>Signaling pathways regulating pluripotency of stem cells;>>Platelet activation;>>Neutrophil extracellular trap formation;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>C-type lectin recep

• Gene Name：
• MAPK1/MAPK3

• Protein Name：
• Mitogen-activated protein kinase 3

• Human Gene Id：
• 5595/5594

• Human Swiss Prot No：
• P27361/P28482

• Mouse Gene Id：
• 26417/26413

• Rat Gene Id：
• 50689/116590

• Rat Swiss Prot No：
• P21708/P63086

• Immunogen：
• Synthesized phospho-peptide around the phosphorylation site of human ERK 1/2 (phospho Thr202/Y204)

• Specificity：
• Phospho-ERK 1/2 (T202/Y204) Polyclonal Antibody detects endogenous levels of ERK 1/2 protein only when phosphorylated at T202 or Y204.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• MAPK3;ERK1;PRKM3;Mitogen-activated protein kinase 3;MAP kinase 3;MAPK 3;ERT2;Extracellular signal-regulated kinase 1;ERK-1;Insulin-stimulated MAP2 kinase;MAP kinase isoform p44;p44-MAPK;Microtubule-associated protein 2 kinase;p

• Molecular Weight(Da)：
• 43kD

• Background：
• The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.,enzyme regulation:Activated by tyrosine phosphorylation in response to insulin and NGF.,function:Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).,PTM:Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinas

• Subcellular Location：
• Cytoplasm . Nucleus. Membrane, caveola . Cell junction, focal adhesion . Autophosphorylation at Thr-207 promotes nuclear localization (PubMed:19060905). PEA15-binding redirects the biological outcome of MAPK3 kinase-signaling by sequestering MAPK3 into the cytoplasm (By similarity). .

• Expression：
• Epithelium,Eye,Hepatoma,Human cervix,Lymph,

Circular RNA circNRIP1 promotes migration and invasion in cervical cancer by sponging miR-629-3p and regulating the PTP4A1/ERK1/2 pathway. Cell Death & Disease Cell Death Dis. 2020 May;11(5):1-15 WB Human HeLa cell,SiHa cell

Effect of cold stress on the MAPK pathway and lipidomics on muscle of Takifugu fasciatus. AQUACULTURE Aquaculture. 2021 Jul;540:736691 WB Fish 1:1500 Muscles

Preventative effects of resveratrol and estradiol on streptozotocin-induced diabetes in ovariectomized mice and the related mechanisms. PLoS One 2018 Oct 01 WB Mouse Skeletal muscle,myocardial tissues

CXCL5, the upregulated chemokine in patients with uterine cervix cancer, in vivo and in vitro contributes to oncogenic potential of Hela uterine cervix cancer cells. BIOMEDICINE & PHARMACOTHERAPY Biomed Pharmacother. 2018 Nov;107:1496 WB Human,Mouse 1:1000 Hela cell-xenografts Hela cell

Novel paeonol derivatives: Design, synthesis and anti-inflammatory activity in vitro and in vivo. BIOORGANIC CHEMISTRY Bioorg Chem. 2020 May;98:103735 WB Mouse RAW264.7 cell

Mesenchymal stem cell‑derived extracellular vesicles promote the in vitro proliferation and migration of breast cancer cells through the activation of the ERK pathway. INTERNATIONAL JOURNAL OF ONCOLOGY Int J Oncol. 2019 May;54(5):1843-1852 WB Human 1:1000 hUC-MSC-EVs

Negative Regulation of Grb10 Interacting GYF Protein 2 on Insulin-Like Growth Factor-1 Receptor Signaling Pathway Caused Diabetic Mice Cognitive Impairment. PLoS One 2014 Sep 30 WB Mouse 1∶1000 hippocampus

CTGF enhances resistance to 5-FU-mediating cell apoptosis through FAK/MEK/ERK signal pathway in colorectal cancer. OncoTargets and Therapy Oncotargets Ther. 2016; 9: 7285–7295 WB Human 1:500 HT-29 cell, SW620 cell

Aβ40 Promotes the Osteoblastic Differentiation of Aortic Valve Interstitial Cells through the RAGE Pathway. Current Medical Science 2020 Oct 29 WB Human 1:500 Acute aortic aortic valve interstitial cells (AVICs)

Zheng, Jianlei, et al. "The effects of YKL-40 on angiogenic potential of HUVECs are partly mediated by syndecan-4." bioRxiv (2020).

SCF/C-KIT signaling modulates tryptase expression in acute myeloid leukemia cells. INTERNATIONAL JOURNAL OF HEMATOLOGY Int J Hematol. 2014 Jun;99(6):750-757 WB Human U937 cell

Dietary antioxidant quercetin overcomes the acquired resistance of Sorafenib in Sorafenib-resistant hepatocellular carcinoma cells through epidermal growth factor receptor signaling inactivation. Zhou Fuqiong WB Mouse，Human 1:1000 Huh7 cell-xenograft Huh7 cell

Angelica Yinzi Alleviates Pruritus-Related Atopic Dermatitis through Skin Repair, Antioxidation, and Balancing Peripheral μ- and κ-opioid Receptors. Evidence-based Complementary and Alternative Medicine Pengtao You IHC Mouse 1 : 200 Skin, lumbar, spinal cord

Effect of the Pulsatilla decoction n-butanol extract on vulvovaginal candidiasis caused by Candida glabrata and on its virulence factors FITOTERAPIA Jiaping Zhang IF,WB Mouse 1:200 vaginal tissue

Bruceantinol works as a CDK2/4/6 inhibitor to inhibit the growth of breast cancer cells CHEMICO-BIOLOGICAL INTERACTIONS Li Sun WB Human MDA-MB-231 cell

Coptidis rhizoma extract alleviates oropharyngeal candidiasis by gC1qR-EGFR/ERK/c-fos axis-induced endocytosis of oral epithelial cells JOURNAL OF ETHNOPHARMACOLOGY Mengyuan Bao WB Human FaDu cell

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