MCM3 (Phospho Ser112) rabbit pAb

    • Catalog No.:YP1398
    • Applications:WB;ELISA;IHC
    • Reactivity:Human;Rat;Mouse;
      • Target:
      • MCM3
      • Fields:
      • >>DNA replication;>>Cell cycle
      • Gene Name:
      • MCM3
      • Protein Name:
      • MCM3 (Ser112)
      • Human Gene Id:
      • 4172
      • Human Swiss Prot No:
      • P25205
      • Mouse Swiss Prot No:
      • P25206
      • Immunogen:
      • Synthesized phosho peptide around human MCM3 (Ser112)
      • Specificity:
      • This antibody detects endogenous levels of Human MCM3 (phospho-Ser112)
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
      • Purification:
      • The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • DNA replication licensing factor MCM3 (EC 3.6.4.12) (DNA polymerase alpha holoenzyme-associated protein P1) (P1-MCM3) (RLF subunit beta) (p102)
      • Observed Band(KD):
      • 89kD
      • Background:
      • The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012],
      • Function:
      • function:Acts as a factor that allows the DNA to undergo a single round of replication per cell cycle. Required for DNA replication and cell proliferation.,miscellaneous:Associated with the replication-specific DNA polymerase alpha.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the MCM family.,similarity:Contains 1 MCM domain.,
      • Subcellular Location:
      • Nucleus . Chromosome . Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses. .
      • Expression:
      • Cervix,Coronary artery,Epithelium,Lymph,Osteosarcoma,Pancre
      • Products Images
      • Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).