Actinin-α1 Polyclonal Antibody

  • Catalog No.:YT0100
  • Applications:WB,ELISA
  • Reactivity:Human,Mouse,Rat
    • Gene Name:
    • ACTN1
    • Protein Name:
    • Alpha-actinin-1
    • Human Gene Id:
    • 87
    • Human Swiss Prot No:
    • P12814
    • Mouse Swiss Prot No:
    • Q7TPR4
    • Immunogen:
    • Synthesized peptide derived from Actinin-α1 . at AA range: 1-80
    • Specificity:
    • Actinin-α1 Polyclonal Antibody detects endogenous levels of Actinin-α1 protein.
    • Formulation:
    • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    • Source:
    • Rabbit
    • Dilution:
    • Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
    • Purification:
    • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    • Concentration:
    • 1 mg/ml
    • Storage Stability:
    • -20°C/1 year
    • Other Name:
    • ACTN1; Alpha-actinin-1; Alpha-actinin cytoskeletal isoform; F-actin cross-linking protein; Non-muscle alpha-actinin-1
    • MolecularWeight(Da):
    • 103058
    • Observed Band(KD):
    • 103
    • Background:
    • actinin alpha 1(ACTN1) Homo sapiens Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
    • Function:
    • function:F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.,similarity:Belongs to the alpha-actinin family.,similarity:Contains 1 actin-binding domain.,similarity:Contains 2 CH (calponin-homology) domains.,similarity:Contains 2 EF-hand domains.,similarity:Contains 4 spectrin repeats.,subcellular location:Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle.,subunit:Homodimer; antiparallel. Interacts with DDN, MYOZ2, PDLIM2, TTID and LPP.,
    • Subcellular Location:
    • stress fiber,ruffle,extracellular region,extracellular space,intracellular,cytoplasm,cytosol,actin filament,plasma membrane,brush border,cell-cell junction,fascia adherens,focal adhesion,Z disc,platelet
    • Expression:
    • Colon,Epithelium,Hepatocyte,Neuroblastoma,Placenta,Platelet,Skin,Tumor of tongue,