Actinin-α2/3 Polyclonal Antibody

    • Catalog No.:YT0102
    • Applications:WB,IHC-p,IF/ICC,ELISA
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • ACTN2/ACTN3
      • Protein Name:
      • Alpha-actinin-2/3
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human Actinin alpha-2/3. AA range:31-80
      • Specificity:
      • Actinin-α2/3 Polyclonal Antibody detects endogenous levels of Actinin-α2/3 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • ACTN2; Alpha-actinin-2; Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein; ACTN3; Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein
      • MolecularWeight(Da):
      • 103854
      • Observed Band(KD):
      • 103
      • Background:
      • actinin alpha 2(ACTN2) Homo sapiens Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013],
      • Function:
      • disease:Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.,function:F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.,similarity:Belongs to the alpha-actinin family.,similarity:Contains 1 actin-binding domain.,similarity:Contains 2 CH (calponin-homology) domains.,similarity:Contains 2 EF-hand domains.,similarity:Contains 4 spectrin repeats.,subcellular location:Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.,subunit:Homodimer; antiparallel. Also forms heterodimers with ACTN3. Interacts with ADAM12, MYOZ1, MYOZ2 and MYOZ3. Interacts via its C-terminal r
      • Subcellular Location:
      • extracellular region,cytosol,cytoskeleton,actin filament,plasma membrane,focal adhesion,Z disc,filopodium,cortical actin cytoskeleton,platelet alpha granule lumen,pseudopodium,dendritic spine,extracellular exosome,
      • Expression:
      • Brain,PNS,Skeletal muscle,
      • Products Images
      • Western Blot analysis of various cells using Actinin-α2/3 Polyclonal Antibody
      • Western Blot analysis of HuvEc cells using Actinin-α2/3 Polyclonal Antibody
      • Immunofluorescence analysis of HeLa cells, using Actinin alpha-2/3 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using Actinin alpha-2/3 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from HepG2, HeLa, and HUVEC cells, using Actinin alpha-2/3 Antibody. The lane on the right is blocked with the synthesized peptide.