• Target：
• AKAP 149

• Gene Name：
• AKAP1

• Protein Name：
• A-kinase anchor protein 1 mitochondrial

• Human Gene Id：
• 8165

• Human Swiss Prot No：
• Q92667

• Mouse Swiss Prot No：
• O08715

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human AKAP1. AA range:281-330

• Specificity：
• AKAP 149 Polyclonal Antibody detects endogenous levels of AKAP 149 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• AKAP1;AKAP149;PRKA1;A-kinase anchor protein 1; mitochondrial;A-kinase anchor protein 149 kDa;AKAP 149;Dual specificity A-kinase-anchoring protein 1;D-AKAP-1;Protein kinase A-anchoring protein 1;PRKA1;Spermatid A-kinase anchor prot

• Observed Band(KD)：
• 90kD

• Background：
• The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to type I and type II regulatory subunits of PKA and anchors them to the mitochondrion. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment. [provided by RefSeq, Jul 2008],

• Function：
• domain:RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.,function:Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane.,similarity:Contains 1 KH domain.,similarity:Contains 1 Tudor domain.,tissue specificity:AKAP149 is highly expressed in prostate and small intestine whereas S-AKAP84 is expressed in kidney, pancreas, liver, lung and brain. AKAP149 is also expressed in colon carcinoma.,

• Subcellular Location：
• Mitochondrion outer membrane . Mitochondrion .

• Expression：
• Isoform 1 is detected in thymus, prostate, testis, ovary, colon and small intestine (PubMed:8769136). Isoform 2 is highly expressed in testis and detected at much lower levels in kidney, pancreas, liver, lung and brain (PubMed:7499250).

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