ALX3 Polyclonal Antibody

    • Catalog No.:YT0207
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • ALX3
      • Gene Name:
      • ALX3
      • Protein Name:
      • Homeobox protein aristaless-like 3
      • Human Gene Id:
      • 257
      • Human Swiss Prot No:
      • O95076
      • Mouse Swiss Prot No:
      • O70137
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human ALX3. AA range:171-220
      • Specificity:
      • ALX3 Polyclonal Antibody detects endogenous levels of ALX3 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • ALX3;Homeobox protein aristaless-like 3;Proline-rich transcription factor ALX3
      • Observed Band(KD):
      • 36kD
      • Background:
      • This gene encodes a nuclear protein with a homeobox DNA-binding domain that functions as a transcriptional regulator involved in cell-type differentiation and development. Preferential methylation of this gene's promoter is associated with advanced-stage neuroblastoma tumors. [provided by RefSeq, Jul 2008],
      • Function:
      • function:Transcriptional regulator with a possible role in patterning of mesoderm during development.,similarity:Belongs to the paired homeobox family.,similarity:Contains 1 homeobox DNA-binding domain.,
      • Subcellular Location:
      • Nucleus .
      • Expression:
      • Brain,Neuroblastoma,
      • Products Images
      • Western Blot analysis of various cells using ALX3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • Immunofluorescence analysis of HeLa cells, using ALX3 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from 293 cells, using ALX3 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).