AMPKα1 Polyclonal Antibody
- Catalog No.:YT0215
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- AMPKα1
- Fields:
- >>FoxO signaling pathway;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Apelin signaling pathway;>>Tight junction;>>Circadian rhythm;>>Thermogenesis;>>Insulin signaling pathway;>>Adipocytokine signaling pathway;>>Oxytocin signaling pathway;>>Glucagon signaling pathway;>>Insulin resistance;>>Non-alcoholic fatty liver disease;>>Alcoholic liver disease;>>Hypertrophic cardiomyopathy;>>Fluid shear stress and atherosclerosis
- Gene Name:
- PRKAA1
- Protein Name:
- 5'-AMP-activated protein kinase catalytic subunit alpha-1
- Human Gene Id:
- 5562
- Human Swiss Prot No:
- Q13131
- Mouse Gene Id:
- 105787
- Mouse Swiss Prot No:
- Q5EG47
- Rat Gene Id:
- 65248
- Rat Swiss Prot No:
- P54645
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human AMPK1. AA range:451-500
- Specificity:
- AMPKα1 Polyclonal Antibody detects endogenous levels of AMPKα1 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- PRKAA1;AMPK1;5'-AMP-activated protein kinase catalytic subunit alpha-1;AMPK subunit alpha-1;Acetyl-CoA carboxylase kinase;ACACA kinase;Hydroxymethylglutaryl-CoA reductase kinase;HMGCR kinase;Tau-protein kinase PRKAA1
- Observed Band(KD):
- 65kD
- Background:
- The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],
- Function:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Binding of AMP results in allosteric activation, inducing phosphorylation on Thr-174 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio.,function:Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic s
- Subcellular Location:
- Cytoplasm . Nucleus . In response to stress, recruited by p53/TP53 to specific promoters. .
- Expression:
- Brain,Intestine,Liver,Mammary gland,Platelet,Testis
The role of the LncRNA-FA2H-2-MLKL pathway in atherosclerosis by regulation of autophagy flux and inflammation through mTOR-dependent signaling. CELL DEATH AND DIFFERENTIATION 2019 Jan 25 WB Human ECs ,SMCs
Guo, Fengxia. "Transcription Factor EB Alleviates Endothelial Cell Inflammation Through NLRP3 In ammasome-Mediated Cell Pyroptosis in Atherosclerosis."
Guo, Fengxia, et al. "Transcription Factor EB Alleviates Endothelial Cell Inflammation Through NLRP3 Inflammasome-Mediated Cell Pyroptosis in Atherosclerosis." (2020).
Liguzinediol potentiates the metabolic remodeling by activating the AMPK/SIRT3 pathway and represses Caspase-3/GSDME-mediated pyroptosis to ameliorate cardiotoxicity Chinese Medicine Zhu Weijie WB Mouse,Rat heart tissue H9C2 cell
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- May 20-2022
- Cell-Based ELISA│解您多样本WB检测之困扰
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Immunofluorescence analysis of rat-lung tissue. 1,AMPKα1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
- Immunofluorescence analysis of rat-lung tissue. 1,AMPKα1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
- Western Blot analysis of mouse-lung cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit(SM-005, Inventbiotech,MN,USA).
- Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,AMPKα1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
- Immunohistochemical analysis of paraffin-embedded Rat-brain tissue. 1,AMPKα1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
- Western Blot analysis of various cells using AMPKα1 Polyclonal Antibody diluted at 1:1000
- Western Blot analysis of HeLa cells using AMPKα1 Polyclonal Antibody diluted at 1:1000
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using AMPK1 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HT29 cells, using AMPK1 Antibody. The lane on the right is blocked with the synthesized peptide.