ATF-7 Polyclonal Antibody
- Catalog No.:YT0390
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse
- Target:
- ATF-7
- Gene Name:
- ATF7
- Protein Name:
- Cyclic AMP-dependent transcription factor ATF-7
- Human Gene Id:
- 11016
- Human Swiss Prot No:
- P17544
- Mouse Gene Id:
- 223922
- Mouse Swiss Prot No:
- Q8R0S1
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human ATF7. AA range:41-90
- Specificity:
- ATF-7 Polyclonal Antibody detects endogenous levels of ATF-7 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- ATF7;ATFA;Cyclic AMP-dependent transcription factor ATF-7;cAMP-dependent transcription factor ATF-7;Activating transcription factor 7;Transcription factor ATF-A
- Observed Band(KD):
- 52kD
- Background:
- function:Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters.,similarity:Belongs to the bZIP family.,similarity:Contains 1 bZIP domain.,similarity:Contains 1 C2H2-type zinc finger.,subunit:Binds DNA as a dimer.,
- Function:
- function:Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters.,similarity:Belongs to the bZIP family.,similarity:Contains 1 bZIP domain.,similarity:Contains 1 C2H2-type zinc finger.,subunit:Binds DNA as a dimer.,
- Subcellular Location:
- Nucleus . Nucleus, nucleoplasm . Chromosome, telomere . Mainly nucleoplasmic. Restricted distribution to the perinuculear region. The sumoylated form locates to the nuclear periphery.; [Isoform 5]: Cytoplasm .
- Expression:
- Expressed in various tissues including heart, brain, placenta, lung and skeletal muscle. Highest levels in skeletal muscle. Lowest in lung and placenta. ; [Isoform 4]: Strongly expressed in skeletal muscle. Also expressed at lower levels in heart and lung.
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- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using ATF-7 Polyclonal Antibody
- Western blot analysis of lysates from HeLa and HUVEC cells, using ATF7 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human Small intestinal stromal tumor. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).