Bcl-2 Polyclonal Antibody
- Catalog No.:YT0470
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat;Chicken
- Target:
- Bcl-2
- Fields:
- >>EGFR tyrosine kinase inhibitor resistance;>>Endocrine resistance;>>Platinum drug resistance;>>NF-kappa B signaling pathway;>>HIF-1 signaling pathway;>>Sphingolipid signaling pathway;>>p53 signaling pathway;>>Autophagy - animal;>>Protein processing in endoplasmic reticulum;>>PI3K-Akt signaling pathway;>>Apoptosis;>>Apoptosis - multiple species;>>Necroptosis;>>Adrenergic signaling in cardiomyocytes;>>Hedgehog signaling pathway;>>Focal adhesion;>>NOD-like receptor signaling pathway;>>JAK-STAT signaling pathway;>>Neurotrophin signaling pathway;>>Cholinergic synapse;>>Estrogen signaling pathway;>>Parathyroid hormone synthesis, secretion and action;>>AGE-RAGE signaling pathway in diabetic complications;>>Amyotrophic lateral sclerosis;>>Pathways of neurodegeneration - multiple diseases;>>Shigellosis;>>Salmonella infection;>>Toxoplasmosis;>>Tuberculosis;>>Hepatitis B;>>Measles;>>Herpes simplex virus 1 infection;>>Epstein-Barr virus infection;>>Human immunodeficiency virus 1 infection;>>Pathw
- Gene Name:
- BCL2
- Protein Name:
- Apoptosis regulator Bcl-2
- Human Gene Id:
- 596
- Human Swiss Prot No:
- P10415
- Mouse Gene Id:
- 12043
- Mouse Swiss Prot No:
- P10417
- Rat Gene Id:
- 24224
- Rat Swiss Prot No:
- P49950
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human BCL-2. AA range:46-95
- Specificity:
- Bcl-2 Polyclonal Antibody detects endogenous levels of Bcl-2 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- BCL2;Apoptosis regulator Bcl-2
- Observed Band(KD):
- 26kD
- Background:
- BCL2, apoptosis regulator(BCL2) Homo sapiens This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016],
- Function:
- disease:A chromosomal aberration involving BCL2 may be a cause of follicular lymphoma (FL) [MIM:151430]; also known as type II chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.,domain:The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1.,function:Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activati
- Subcellular Location:
- Mitochondrion outer membrane ; Single-pass membrane protein . Nucleus membrane ; Single-pass membrane protein . Endoplasmic reticulum membrane ; Single-pass membrane protein . Cytoplasm .
- Expression:
- Expressed in a variety of tissues.
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Design, Synthesis, and Apoptosis-Promoting Effect Evaluation of Rhopaladins’ Analog 4-Arylidene-5-Oxopyrrolidine Derivatives WB Human 1:1000 /HepG2 cell, 7402 cell
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Melatonin treatment improves human umbilical cord mesenchymal stem cell therapy in a mouse model of type II diabetes mellitus via the PI3K/AKT signaling pathway. Stem Cell Research & Therapy2022 Dec;13(1):1-15. Human,Mouse 1:1200 liver tissue hUC-MSC
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The Protective Effect of Aromatase on NSC-34 Cells with Stably Expressed hSOD1-G93A. NEUROSCIENCE Neuroscience. 2019 Jul;411:37 WB Human 1:500 hSOD1-G93A cell
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Effect of annexin A7 suppression on the apoptosis of gastric cancer cells. MOLECULAR AND CELLULAR BIOCHEMISTRY Mol Cell Biochem. 2017 May;429(1):33-43 WB Human,Mouse BGC823 cell-xenograft BGC823 cell
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Emodin suppresses the migration and invasion of melanoma cells. BIOLOGICAL & PHARMACEUTICAL BULLETIN 2021 Mar 17 WB Mouse,Human 1 : 500 B16F10 Melanoma Cells, A375 Melanoma Cells
The modified Yi qi decoction protects cardiac ischemia-reperfusion induced injury in rats. BMC Complementary and Alternative Medicine 2017 Jun 21 WB Rat 1:1000 Myocardial tissues (AAR tissue)
Exosome-mediated microRNA transfer plays a role in radiation-induced bystander effect. RNA Biology 2016 Jan 12 WB Human MRC5 cell
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HnRNP-L mediates bladder cancer progression by inhibiting apoptotic signaling and enhancing MAPK signaling pathways. Oncotarget Oncotarget. 2017 Feb 21; 8(8): 13586–13599 WB Human UM-UC-3 cell,EJ cell
MiR-21 is involved in radiation-induced bystander effects. RNA Biology 2015 Jan 26 WB MRC-5 cell
Protective effects of crocetin pretreatment on myocardial injury in an ischemia/reperfusion rat model. EUROPEAN JOURNAL OF PHARMACOLOGY Eur J Pharmacol. 2014 Oct;741:290 IHC Rat 1:150 heart
Polycyclic aromatic hydrocarbons are associated with increased risk of chronic obstructive pulmonary disease during haze events in China. SCIENCE OF THE TOTAL ENVIRONMENT 2016 Sep 07 WB Human 1:1000 BEAS-2B cell
The antitumor effect of hinesol, extract from Atractylodes lancea (Thunb.) DC. by proliferation, inhibition, and apoptosis induction via MEK/ERK and NF‐κB pathway in non–small cell lung cancer cell lines A549 and NCI‐H1299. JOURNAL OF CELLULAR BIOCHEMISTRY 2019 Jul 24 WB Human A549 cell
Hydrogen protects against chronic intermittent hypoxia induced renal dysfunction by promoting autophagy and alleviating apoptosis. LIFE SCIENCES Life Sci. 2019 May;225:46 WB Rat kidney
Resveratrol prevents chronic intermittent hypoxia-induced cardiac hypertrophy by targeting the PI3K/AKT/mTOR pathway. LIFE SCIENCES Life Sci. 2019 Sep;233:116748 WB Rat 1:1000 Myocardial tissues
Vangl2 is essential for myocardial remodeling activated by Wnt/JNK signaling. EXPERIMENTAL CELL RESEARCH Exp Cell Res. 2018 Apr;365:33 WB Rat cardiomyocytes
Cancer Stem Cells are Regulated by STAT3 Signalling in Wilms Tumour. Journal of Cancer J Cancer. 2018; 9(8): 1486–1499 WB Human,Mouse 1:1000 G401-Xenografts G401 cell
Long noncoding RNA LINC00152 promotes cell proliferation through competitively binding endogenous miR‐125b with MCL‐1 by regulating mitochondrial apoptosis pathways in ovarian cancer. Cancer Medicine Cancer Med-Us. 2018 Sep;7(9):4530-4541 WB Human,Mouse xenograft SKOV3 cell, A2780 cell
Reversing platinum resistance in ovarian cancer multicellular spheroids by targeting Bcl-2. OncoTargets and Therapy Oncotargets Ther. 2019; 12: 897–906 WB Human SKOV-3 cell, OVCAR-3 cell
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Atrazine Causes Autophagy- and Apoptosis-Related Neurodegenerative Effects in Dopaminergic Neurons in the Rat Nigrostriatal Dopaminergic System. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2015 Jun 12 WB Rat ventral midbrain
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LC3-II may mediate ATR-induced mitophagy in dopaminergic neurons through SQSTM1/p62 pathway. ACTA BIOCHIMICA ET BIOPHYSICA SINICA 2018 Oct 01 WB Rat 1:1000 substantia nigra (SNpc) PC-12 cell
Rce1 expression in renal cell carcinoma and its regulatory effect on 786-O cell apoptosis through endoplasmic reticulum stress. ACTA BIOCHIMICA ET BIOPHYSICA SINICA 2017 Mar 01 WB Human 1:2000 renal cancer tissues 786-O cell
Protective effect of luteolin on cigarette smoke extract‑induced cellular toxicity and apoptosis in normal human bronchial epithelial cells via the Nrf2 pathway. ONCOLOGY REPORTS Oncol Rep. 2014 Apr;31(4):1855-1862 WB Human NHBE cell
Total Flavonoids from Carya cathayensis Sarg. Leaves Alleviate H9c2 Cells Hypoxia/Reoxygenation Injury via Effects on miR-21 Expression, PTEN/Akt, and the Bcl-2/Bax Pathway. Evidence-based Complementary and Alternative Medicine Evid-Based Compl Alt. 2018;2018:8617314 WB Rat 1:1000 H9c2 cell
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VX-680 induces p53-mediated apoptosis in human cholangiocarcinoma cells. ANTI-CANCER DRUGS Anti-Cancer Drug. 2018 Nov;29(10):1004-1010 WB Human 1 : 1000 HCCC-9810 cell
Combined effects of aspirin and vitamin D3 on two OSCC cell lines: a preliminary study. BIOTECHNOLOGY LETTERS 2018 Jan 18 WB Human CAL-27 cell,SCC-15 cell
Down-regulation of FoxM1 by thiostrepton or small interfering RNA inhibits proliferation, transformation ability and angiogenesis, and induces apoptosis of nasopharyngeal carcinoma cells. International Journal of Clinical and Experimental Pathology Int J Clin Exp Patho. 2014; 7(9): 5450–5460 WB Human Nasopharyngeal carcinoma (NPC) cell
Synergistic Apoptotic Effect of D-Fraction From Grifola frondosa and Vitamin C on Hepatocellular Carcinoma SMMC-7721 Cells:. INTEGRATIVE CANCER THERAPIES 2016 May 05 WB Human SMMC-7721 cell
Exosome-derived microRNA-29c Induces Apoptosis of BIU-87 Cells by Down Regulating BCL-2 and MCL-1. Asian Pacific Journal of Cancer Prevention 2014 Apr 30 IHC Human Bladder Cancer tissue
Effect of metformin on apoptosis, cell cycle arrest migration and invasion of A498 cells. Molecular Medicine Reports 2014 Mar 31 WB Human A498 cell
Protective effects of tanshinone IIA sodium sulfonate on ischemia-reperfusion-induced myocardial injury in rats. Iranian Journal of Basic Medical Sciences Iran J Basic Med Sci. 2017 Mar; 20(3): 308–315 WB Rat 1:1000 Myocardial tissues
Apoptosis triggered by pyropheophorbide-α methyl ester-mediated photodynamic therapy in a giant cell tumor in bone. LASER PHYSICS Laser Phys. 2014 May;24(6):066002 WB Human SGC-0404 cell
miR-2954 Inhibits PI3K Signaling and Induces Autophagy and Apoptosis in Myocardium Selenium Deficiency. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 2018 Nov 21 WB Chicken 1:500 cardiomyocytes
Effects of Ovariectomy in an hSOD1-G93A Transgenic Mouse Model of Amyotrophic Lateral Sclerosis (ALS). MEDICAL SCIENCE MONITOR Med Sci Monitor. 2018; 24: 678–686 WB Mouse 1: 500 spinal cord
Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang Improves Cardiac Function after Myocardial Infarction in Rats. Evidence-based Complementary and Alternative Medicine 2020 Oct 09 WB Rat 1 : 1000 Myocardial tissue
Mitochondrial separation protein inhibitor inhibits cell apoptosis in rat lungs during intermittent hypoxia. Experimental and Therapeutic Medicine 2019 Mar 01 WB Human,Rat WRTL1 cell
Fang, Zhiqing, et al. "Nitidine chloride induces apoptosis and inhibits tumor cell proliferation via suppressing ERK signaling pathway in renal cancer." Food and chemical toxicology 66 (2014): 210-216.
Fang, Zhiqing, et al. "Simvastatin inhibits renal cancer cell growth and metastasis via AKT/mTOR, ERK and JAK2/STAT3 pathway." PloS one 8.5 (2013): e62823.
N′-[(3-[benzyloxy] benzylidene]-3, 4, 5-trihydroxybenzohydrazide (1) protects mice against colitis induced by dextran sulfate sodium through inhibiting NFκB/IL-6/STAT3 pathway." Biochemical and biophysical research communications 477.2 (2016): 290-296.
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Astragaloside IV Attenuates the Myocardial Injury Caused by Adriamycin by Inhibiting Autophagy. Frontiers in Pharmacology Front Pharmacol. 2021 May;0:1289 WB Rat 1 : 1500 Myocardial tissue
Flavonoid-Rich Extract of Oldenlandia diffusa (Willd.) Roxb. Inhibits Gastric Cancer by Activation of Caspase-Dependent Mitochondrial Apoptosis Chinese Journal of Integrative Medicine Qiu-lan Wang WB Human
Establishment of fingerprint and mechanism of anti-myocardial ischemic effect of Syringa pinnatifolia BIOMEDICAL CHROMATOGRAPHY Peifeng Xue WB Mouse
Tubeimoside I Ameliorates Doxorubicin-Induced Cardiotoxicity by Upregulating SIRT3 Oxidative Medicine and Cellular Longevity Congxin Huang WB Mouse heart tissue H9c2 cells
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TAK-715 alleviated IL-1β-induced apoptosis and ECM degradation in nucleus pulposus cells and attenuated intervertebral disc degeneration ex vivo and in vivo ARTHRITIS RESEARCH & THERAPY Lin Huang IHC,WB Rat disc samples,nucleus pulposus cells (NPCs)samples
MicroRNA-155 suppressed cholesterol-induced matrix degradation, pyroptosis and apoptosis by targeting RORα in nucleus pulposus cells CELLULAR SIGNALLING Wei Ye IHC Rat nucleus pulposus (NP) tissue
Comparison of two kinds of Agrocybe cylindracea polysaccharides: structural characteristic and antitumor activity. An-jun Liu WB Human MGC-803 cell
Preventive effect of LCZ696 on hypoxic pulmonary hypertension in rats via regulating the PI3K/AKT signaling pathway. Ya-Feng Wang WB Rat 1:1000 Lung tissue
Dangshen Huangjiu exerts prevention of gastric mucosal injury and inhibits Akt/NF-κB signaling. Fangdi Hu WB Rat gastric mucosal tissues
Celastrol attenuates diabetic nephropathy by upregulating SIRT1-mediated inhibition of EZH2 related wnt/β-catenin signaling. Ruchun Yang WB Mouse podocytes, MPC5 cell
Indisulam exerts anticancer effects via modulation of transcription, translation and alternative splicing on human cervical cancer cells. Cuixia Di WB Human 1:1000 HeLa cell
Itaconic acid induces angiogenesis and suppresses apoptosis via Nrf2/autophagy to prolong the survival of multi-territory perforator flaps. Liming Qing WB Rat 1:1000 flap tissue
Dietary antioxidant quercetin overcomes the acquired resistance of Sorafenib in Sorafenib-resistant hepatocellular carcinoma cells through epidermal growth factor receptor signaling inactivation. Zhou Fuqiong WB Mouse,Human 1:1000 Huh7 cell-xenograft Huh7 cell
Banxia-Houpu decoction inhibits iron overload and chronic intermittent hypoxia-induced neuroinflammation in mice. JOURNAL OF ETHNOPHARMACOLOGY Yashuo Zhao WB Mouse hippocampal
Role of Txnrd3 in NiCl2-induced kidney cell apoptosis in mice: Potential therapeutic effect of melatonin. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Ziwei Zhang WB Mouse 1:2000 kidney
Protective effect of cornuside on OGD/R injury in SH-SY5Y cells and its underlying mechanism. BRAIN RESEARCH Jianwei Gong WB Human SH-SY5Y cell
L-Theanine delays D-galactose-induced senescence by regulating the cell cycle and inhibiting apoptosis in rat intestinal cells JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE Wei Xu WB Rat 1:2000 IEC-6 cell
The role of vitamin D through SphK1/S1P in the regulation of MS progression JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY Zhen Wang WB Rat PC12 cell
maresin2 fine-tunes ULK1 O-GlcNAcylation to improve post myocardial infarction remodeling EUROPEAN JOURNAL OF PHARMACOLOGY Jingjing Zhang WB Mouse,Rat 1:1000 heart tissue neonatal rat cardiomyocytes (NRCMs)
Proteomic analysis reveals that cigarette smoke exposure diminishes ovarian reserve in mice by disrupting the CREB1-mediated ovarian granulosa cell proliferation-apoptosis balance ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Mengting Xu WB Mouse,Human 1:1000 ovarian tissue KGN cell
Roflupram alleviates autophagy defects and reduces mutant hSOD1-induced motor neuron damage in cell and mouse models of amyotrophic lateral sclerosis NEUROPHARMACOLOGY Di Huo WB Mouse 1:1000 spinal cord tissue NSC34 cell
Synthesis of peiminine derivatives and its effect on A549 lung cancer-bearing mice Chemical Biology & Drug Design Xin Jin WB Mouse A549 cell-Xenograft
L-theanine protects against D-galactose-induced aging in L6 skeletal muscle cells Journal of Functional Foods Min Long WB Rat 1:2000 L6 cell
ADPN Regulates Oxidative Stress-Induced Follicular Atresia in Geese by Modulating Granulosa Cell Apoptosis and Autophagy INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Yan Zheng WB Goose 1:1000 follicular tissue
Lung decellularized matrix-derived 3D spheroids: Exploring silicosis through the impact of the Nrf2/Bax pathway on myofibroblast dynamics Heliyon Wenming Xue IF,WB Mouse 1:1000 NIH/3T3 cell
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- Banxia-Houpu decoction inhibits iron overload and chronic intermittent hypoxia-induced neuroinflammation in mice. JOURNAL OF ETHNOPHARMACOLOGY Yashuo Zhao WB Mouse hippocampal
- Xiao, Wei, et al. "Notch signaling plays a crucial role in cancer stem-like cells maintaining stemness and mediating chemotaxis in renal cell carcinoma." Journal of Experimental & Clinical Cancer Research 36.1 (2017): 41.
- Liu, Jing-Cai, et al. "Di (2-ethylhexyl) phthalate exposure impairs meiotic progression and DNA damage repair in fetal mouse oocytes in vitro." Cell death & disease 8.8 (2017): e2966.
- Xu, Shuai, et al. "Exosome-mediated microRNA transfer plays a role in radiation-induced bystander effect." RNA biology12.12 (2015): 1355-1363.
- Bai, Ding-Ping, et al. "Zinc oxide nanoparticles induce apoptosis and autophagy in human ovarian cancer cells." International journal of nanomedicine 12 (2017): 6521.
- Liu, Yanmei, et al. "Cancer Stem Cells are Regulated by STAT3 Signalling in Wilms Tumour." Journal of Cancer 9.8 (2018): 1486.
- Chen, Puxiang, et al. "Long noncoding RNA LINC00152 promotes cell proliferation through competitively binding endogenous miR‐125b with MCL‐1 by regulating mitochondrial apoptosis pathways in ovarian cancer." Cancer medicine 7.9 (2018): 4530-4541.
- Yang, Ya’nan, et al. "Reversing platinum resistance in ovarian cancer multicellular spheroids by targeting Bcl-2." OncoTargets and therapy 12 (2019): 897.
- Fang, Zhiqing, et al. "Simvastatin inhibits renal cancer cell growth and metastasis via AKT/mTOR, ERK and JAK2/STAT3 pathway." PloS one 8.5 (2013): e62823.
- Jiang, Ruibin, et al. "Total Flavonoids from Carya cathayensis Sarg. Leaves Alleviate H9c2 Cells Hypoxia/Reoxygenation Injury via Effects on miR-21 Expression, PTEN/Akt, and the Bcl-2/Bax Pathway." Evidence-Based Complementary and Alternative Medicine 2018 (2018).
- Pan, Yun, et al. "Protective effects of tanshinone IIA sodium sulfonate on ischemia-reperfusion-induced myocardial injury in rats." Iranian journal of basic medical sciences 20.3 (2017): 308.
- Wang, Ai-Li, et al. "Glutamine ameliorates intestinal ischemia-reperfusion Injury in rats by activating the Nrf2/Are signaling pathway." International journal of clinical and experimental pathology 8.7 (2015): 7896.
- Western blot analysis of lysates from 1)mouse-liver, 2)mouse-brain, 3)mouse-lung, 4)mcf-7 cells, (Green) primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(Immunoway:RS23920)was diluted at 1:10000, 37° 1hour. (Red) Actin β Monoclonal Antibody(5B7) (Immunoway:YM3028) antibody was diluted at 1:5000 as loading control, 4° over night,Dylight 680 secondary antibody(Immunoway:RS23710)was diluted at 1:10000, 37° 1hour.
- Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit(SM-005, Inventbiotech,MN,USA).
- Immunofluorescence analysis of HepG2 cells, using BCL-2 Antibody. The picture on the right is blocked with the synthesized peptide.
- Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using BCL-2 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from K562 cells, using BCL-2 Antibody. The lane on the right is blocked with the synthesized peptide.