Caldesmon Polyclonal Antibody

  • Catalog No.：YT0608
  • Applications：WB;IHC;IF;IP;ELISA
  • Reactivity：Human;Mouse;Rat
    • Target：
    • Caldesmon
    • Fields：
    • >>Vascular smooth muscle contraction
    • Gene Name：
    • CALD1
    • Protein Name：
    • Caldesmon
    • Human Gene Id：
    • 800
    • Human Swiss Prot No：
    • Q05682
    • Immunogen：
    • The antiserum was produced against synthesized peptide derived from human Caldesmon. AA range:744-793
    • Specificity：
    • Caldesmon Polyclonal Antibody detects endogenous levels of Caldesmon protein.
    • Formulation：
    • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    • Source：
    • Polyclonal, Rabbit,IgG
    • Dilution：
    • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
    • Purification：
    • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    • Concentration：
    • 1 mg/ml
    • Storage Stability：
    • -15°C to -25°C/1 year(Do not lower than -25°C)
    • Other Name：
    • CALD1;CAD;CDM;Caldesmon;CDM
    • Observed Band(KD)：
    • 80kD
    • Background：
    • This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008],
    • Function：
    • domain:The N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form.,function:Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping.,PTM:In non-muscle cells, phosphorylation by CDC2 during mit
    • Subcellular Location：
    • Cytoplasm, cytoskeleton . Cytoplasm, myofibril . Cytoplasm, cytoskeleton, stress fiber . On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle). .
    • Expression：
    • High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.

    Attributes

    • Code：
    • YT0608
    • Size
    • 40μL / 100μL / 200μL
    • Price$：
    • 150 / 280 / 450
    • Delivery：
    • 2024-11-25
    Order

    Related Products