c-FLIP Polyclonal Antibody

    • Catalog No.：YT0877
    • Applications：WB;IHC;IF;ELISA
    • Reactivity：Human;Mouse
      • Target：
      • c-FLIP
      • Fields：
      • >>NF-kappa B signaling pathway;>>Autophagy - animal;>>Apoptosis;>>Necroptosis;>>TNF signaling pathway;>>Chagas disease;>>Hepatitis C
      • Gene Name：
      • CFLAR
      • Protein Name：
      • CASP8 and FADD-like apoptosis regulator
      • Human Gene Id：
      • 8837
      • Human Swiss Prot No：
      • O15519
      • Mouse Swiss Prot No：
      • O35732
      • Immunogen：
      • The antiserum was produced against synthesized peptide derived from human in ternal CFLAR. AA range:181-230
      • Specificity：
      • c-FLIP Polyclonal Antibody detects endogenous levels of c-FLIP protein.
      • Formulation：
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source：
      • Polyclonal, Rabbit,IgG
      • Dilution：
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
      • Purification：
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration：
      • 1 mg/ml
      • Storage Stability：
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name：
      • CFLAR;CASH;CASP8AP1;CLARP;MRIT;CASP8 and FADD-like apoptosis regulator;Caspase homolog;CASH;Caspase-eight-related protein;Casper;Caspase-like apoptosis regulatory protein;CLARP;Cellular FLICE-like inhibitory protein;c-FLIP;FAD
      • Observed Band(KD)：
      • 55kD
      • Background：
      • The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011],
      • Function：
      • domain:The caspase domain lacks the active sites residues involved in catalysis.,function:Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.,induction:Repressed by IL-2 after TCR stimulation, during progression to the S-phase of the cell cycle.,PTM:Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12.,similarity:Belongs to the peptidase C14A family.,similarity:Contains
      • Subcellular Location：
      • cytoplasm,cytosol,death-inducing signaling complex,CD95 death-inducing signaling complex,membrane raft,ripoptosome,
      • Expression：
      • Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.
      • Products Images
      • Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

      Attributes

      • Code：
      • YT0877
      • Size
      • 40μL / 100μL / 200μL
      • Price$：
      • 150 / 280 / 450
      • Delivery：
      • 2024-12-23
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