FAK Polyclonal Antibody
- Catalog No.:YT1659
- Applications:IF;WB;IHC;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- FAK
- Fields:
- >>Endocrine resistance;>>ErbB signaling pathway;>>Chemokine signaling pathway;>>PI3K-Akt signaling pathway;>>Axon guidance;>>VEGF signaling pathway;>>Focal adhesion;>>Leukocyte transendothelial migration;>>Regulation of actin cytoskeleton;>>Growth hormone synthesis, secretion and action;>>Bacterial invasion of epithelial cells;>>Shigellosis;>>Yersinia infection;>>Amoebiasis;>>Human cytomegalovirus infection;>>Human papillomavirus infection;>>Human immunodeficiency virus 1 infection;>>Pathways in cancer;>>Transcriptional misregulation in cancer;>>Proteoglycans in cancer;>>Chemical carcinogenesis - reactive oxygen species;>>Small cell lung cancer;>>Lipid and atherosclerosis;>>Fluid shear stress and atherosclerosis
- Gene Name:
- PTK2
- Protein Name:
- Focal adhesion kinase 1
- Human Gene Id:
- 5747
- Human Swiss Prot No:
- Q05397
- Mouse Gene Id:
- 14083
- Mouse Swiss Prot No:
- P34152
- Rat Gene Id:
- 25614
- Rat Swiss Prot No:
- O35346
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human FAK. AA range:809-858
- Specificity:
- FAK Polyclonal Antibody detects endogenous levels of FAK protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- PTK2;FAK;FAK1;Focal adhesion kinase 1;FADK 1;Focal adhesion kinase-related nonkinase;FRNK;Protein phosphatase 1 regulatory subunit 71;PPP1R71;Protein-tyrosine kinase 2;p125FAK;pp125FAK
- Observed Band(KD):
- 120kD
- Background:
- protein tyrosine kinase 2(PTK2) Homo sapiens This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only four of them have been determined. [provided by RefSeq, Oct 2015],
- Function:
- catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,domain:The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.,domain:The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.,function:Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.,PTM:Phosphorylated on 6 tyrosine residues upon activatio
- Subcellular Location:
- Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Nucleus. Cytoplasm, cytoskeleton, cilium basal body . Constituent of focal adhesions. Detected at microtubules.
- Expression:
- Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level). Ubiquitous. Expressed in epithelial cells (at protein level) (PubMed:31630787).
Albumin Fusion at the N-Terminus or C-Terminus of HM-3 Leads to Improved Pharmacokinetics and Bioactivities. Biomedicines Biomedicines. 2021 Sep;9(9):1084 WB Mouse Murine melanoma cells (B16F10)
HM-3-HSA exhibits potent anti-angiogenesis and antitumor activity in hepatocellular carcinoma. EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES Eur J Pharm Sci. 2021 Sep;:106017 WB Human HUVECs
Dissecting the roles of Ephrin-A3 in malignant peripheral nerve sheath tumor by TALENs. ONCOLOGY REPORTS Oncol Rep. 2015 Jul;34(1):391-398 WB Human ST88-14 cell, sNF96.2 cell
Exosomes derived from 5-fluorouracil-resistant colon cancer cells are enriched in GDF15 and can promote angiogenesis. Journal of Cancer J Cancer. 2020; 11(24): 7116–7126 WB Human 1 : 1000 HCT-15/FU exosome
Lu, Jian, et al. "Role of LKB1 in migration and invasion of Cr (VI)-transformed human bronchial epithelial Beas-2B cells." Anti-cancer drugs 29.7 (2018): 660-673.
Integrin β4 Regulates Cell Migration of Lung Adenocarcinoma Through FAK Signaling MOLECULAR BIOTECHNOLOGY Zhang Shusen WB Human 1:1000 PC9 cell,NCIH1975 cell,A549 cell
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- Antibody-FAQs
- Products Images
- Immunofluorescence analysis of Hela cell. 1,FAK Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
- Immunofluorescence analysis of human-liver tissue. 1,FAK Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
- Immunofluorescence analysis of human-lung tissue. 1,FAK Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
- Immunofluorescence analysis of rat-kidney tissue. 1,FAK Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
- Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit(SM-005, Inventbiotech,MN,USA).
- Western blot analysis of HELA KB SH-SY5Y 293T 3T3 lysis using FAK antibody. Antibody was diluted at 1:2000
- Western blot analysis of customer's lysis using FAK antibody. Antibody was diluted at 1:2000
- Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using FAK Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from MCF-7 and HUVEC cells, using FAK Antibody. The lane on the right is blocked with the synthesized peptide.