FoxK1 Polyclonal Antibody
- Catalog No.:YT1753
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse
- Target:
- FoxK1
- Gene Name:
- FOXK1
- Protein Name:
- Forkhead box protein K1
- Human Gene Id:
- 221937
- Human Swiss Prot No:
- P85037
- Mouse Gene Id:
- 17425
- Mouse Swiss Prot No:
- P42128
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human FOXK1. AA range:681-730
- Specificity:
- FoxK1 Polyclonal Antibody detects endogenous levels of FoxK1 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- FOXK1;MNF;Forkhead box protein K1;Myocyte nuclear factor;MNF
- Observed Band(KD):
- 78kD
- Background:
- function:Transcriptional regulator that binds to the upstream enhancer region (CCAC box) of myoglobin gene. Has a role in myogenic differentiation and in remodeling processes of adult muscles that occur in response to physiological stimuli.,PTM:Phosphorylated.,similarity:Contains 1 FHA domain.,similarity:Contains 1 fork-head DNA-binding domain.,subunit:Interacts with SIN3B to form a complex which represses transcription.,tissue specificity:Expressed both developing and adult tissues. In adults, significant expression is seen in tumors of the brain, colon and lymph node.,
- Function:
- function:Transcriptional regulator that binds to the upstream enhancer region (CCAC box) of myoglobin gene. Has a role in myogenic differentiation and in remodeling processes of adult muscles that occur in response to physiological stimuli.,PTM:Phosphorylated.,similarity:Contains 1 FHA domain.,similarity:Contains 1 fork-head DNA-binding domain.,subunit:Interacts with SIN3B to form a complex which represses transcription.,tissue specificity:Expressed both developing and adult tissues. In adults, significant expression is seen in tumors of the brain, colon and lymph node.,
- Subcellular Location:
- Nucleus . Cytoplasm . Translocation to the nucleus is regulated by phosphorylation: phosphorylation by GSK3 (GSK3A or GSK3B) promotes interaction with 14-3-3 proteins and sequestration in the cytoplasm. Dephosphorylation promotes translocation to the nucleus (By similarity). Accumulates in the nucleus upon viral infection (PubMed:25852164). .
- Expression:
- Expressed both developing and adult tissues (PubMed:15289879). In adults, significant expression is seen in tumors of the brain, colon and lymph node (PubMed:15289879).
FOXK1 upregulation is correlated with tumor progression and tumor associated macrophages infiltration in renal cell carcinoma. MOLECULAR CARCINOGENESIS Jianqing Ye WB,IHC Human RCC(renal cell carcinoma) tumor tissues 769-P cell,786-O cell
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- Antibody-FAQs
- Products Images
- Western Blot analysis of Jurkat cells using FoxK1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western blot analysis of lysates from Jurkat cells, using FOXK1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from K562 cells using FOXK1 antibody.
- Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200