• Target：
• Frizzled-10

• Fields：
• >>mTOR signaling pathway;>>Wnt signaling pathway;>>Hippo signaling pathway;>>Signaling pathways regulating pluripotency of stem cells;>>Melanogenesis;>>Cushing syndrome;>>Alzheimer disease;>>Pathways of neurodegeneration - multiple diseases;>>Human papillomavirus infection;>>Pathways in cancer;>>Proteoglycans in cancer;>>Basal cell carcinoma;>>Breast cancer;>>Hepatocellular carcinoma;>>Gastric cancer

• Gene Name：
• FZD10

• Protein Name：
• Frizzled-10

• Human Gene Id：
• 11211

• Human Swiss Prot No：
• Q9ULW2

• Mouse Gene Id：
• 93897

• Mouse Swiss Prot No：
• Q8BKG4

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human FZD10. AA range:135-184

• Specificity：
• Frizzled-10 Polyclonal Antibody detects endogenous levels of Frizzled-10 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• FZD10;Frizzled-10;Fz-10;hFz10;FzE7;CD antigen CD350

• Observed Band(KD)：
• 60kD

• Background：
• This gene is a member of the frizzled gene family. Members of this family encode 7-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. Using array analysis, expression of this intronless gene is significantly up-regulated in two cases of primary colon cancer. [provided by RefSeq, Jul 2008],

• Function：
• domain:Lys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway.,domain:The FZ domain is involved in binding with Wnt ligands.,function:Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphog

• Subcellular Location：
• Cell membrane ; Multi-pass membrane protein .

• Expression：
• Highest levels in the placenta and fetal kidney, followed by fetal lung and brain. In adult brain, abundantly expressed in the cerebellum, followed by cerebral cortex, medulla and spinal cord; very low levels in total brain, frontal lobe, temporal lobe and putamen. Weak expression detected in adult brain, heart, lung, skeletal muscle, pancreas, spleen and prostate.

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