GK2 Polyclonal Antibody
- Catalog No.:YT1912
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse
- Target:
- GK2
- Fields:
- >>Glycerolipid metabolism;>>Metabolic pathways;>>PPAR signaling pathway
- Gene Name:
- GK2
- Protein Name:
- Glycerol kinase 2
- Human Gene Id:
- 2712
- Human Swiss Prot No:
- Q14410
- Mouse Swiss Prot No:
- Q9WU65
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human GK2. AA range:281-330
- Specificity:
- GK2 Polyclonal Antibody detects endogenous levels of GK2 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- GK2;GKP2;GKTA;Glycerol kinase 2;GK 2;Glycerokinase 2;ATP:glycerol 3-phosphotransferase 2;Glycerol kinase; testis specific 2
- Observed Band(KD):
- 61kD
- Background:
- catalytic activity:ATP + glycerol = ADP + sn-glycerol 3-phosphate.,function:Key enzyme in the regulation of glycerol uptake and metabolism.,pathway:Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.,similarity:Belongs to the FGGY kinase family.,subcellular location:In sperm the majority of the enzyme is bound to mitochondria.,
- Function:
- catalytic activity:ATP + glycerol = ADP + sn-glycerol 3-phosphate.,function:Key enzyme in the regulation of glycerol uptake and metabolism.,pathway:Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.,similarity:Belongs to the FGGY kinase family.,subcellular location:In sperm the majority of the enzyme is bound to mitochondria.,
- Subcellular Location:
- Mitochondrion outer membrane ; Single-pass type IV membrane protein . Cytoplasm . In sperm the majority of the enzyme is bound to mitochondria. .
- Expression:
- Testis-specific (PubMed:33536340). Expressed in the midpiece of spermatozoa (PubMed:28852571).
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- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using GK2 Polyclonal Antibody
- Western Blot analysis of Jurkat cells using GK2 Polyclonal Antibody
- Western blot analysis of lysates from HeLa, 293, and Jurkat cells, using GK2 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).