• Target：
• HIF-1α

• Fields：
• >>HIF-1 signaling pathway;>>Mitophagy - animal;>>Autophagy - animal;>>Th17 cell differentiation;>>Thyroid hormone signaling pathway;>>Kaposi sarcoma-associated herpesvirus infection;>>Pathways in cancer;>>Proteoglycans in cancer;>>Chemical carcinogenesis - reactive oxygen species;>>Renal cell carcinoma;>>Central carbon metabolism in cancer;>>Choline metabolism in cancer;>>PD-L1 expression and PD-1 checkpoint pathway in cancer

• Gene Name：
• HIF1A

• Protein Name：
• Hypoxia-inducible factor 1-alpha

• Human Gene Id：
• 3091

• Human Swiss Prot No：
• Q16665

• Mouse Gene Id：
• 15251

• Mouse Swiss Prot No：
• Q61221

• Rat Gene Id：
• 29560

• Rat Swiss Prot No：
• O35800

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human HIF-1alpha. AA range:328-377

• Specificity：
• HIF-1α Polyclonal Antibody detects endogenous levels of HIF-1α protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IF 1:50-200 WB 1:500 - 1:2000.IP 1:200 IHC 1:100 - 1:300. ELISA: 1:40000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• HIF1A;BHLHE78;MOP1;PASD8;Hypoxia-inducible factor 1-alpha;HIF-1-alpha;HIF1-alpha;ARNT-interacting protein;Basic-helix-loop-helix-PAS protein MOP1;Class E basic helix-loop-helix protein 78;bHLHe78;Member of PAS protein 1;PAS doma

• Observed Band(KD)：
• 92-130kD

• Background：
• hypoxia inducible factor 1 alpha subunit(HIF1A) Homo sapiens This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011],

• Function：
• domain:Contains two independent C-terminal transactivation domains, NTAD and CTAD, which function synergistically. Their transcriptional activity is repressed by an intervening inhibitory domain (ID).,function:Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Acti

• Subcellular Location：
• Cytoplasm . Nucleus . Nucleus speckle . Colocalizes with HIF3A in the nucleus and speckles (By similarity). Cytoplasmic in normoxia, nuclear translocation in response to hypoxia (PubMed:9822602). .

• Expression：
• Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. A higher level expression seen in pituitary tumors as compared to the pituitary gland.

Quxie Capsule Alleviates Colitis-associated Colorectal Cancer Through Modulating the Gut Microbiota and Suppressing A. fumigatus-induced Aerobic Glycolysis

Preliminary verification of the anti-hypoxia mechanism of Gentiana straminea maxim based on UPLC-triple TOF MS/MS and network pharmacology

Effect of Enterococcus faecalis on osteoclastogenesis under cobalt-mimicked hypoxia in vitro MICROBIAL PATHOGENESIS Fengyi Zhou, Xin Li, Xiaochi Chang, Zhihao Geng, Wenjing Hao, Jing Deng, Hai Ming Wong, Shuai Wang WB Mouse BMMs

circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop WB Human 1:1000 /SW620, HCT116 Cells

Integrating Network Pharmacology and Experimental Validation to Explore the Key Mechanism of Gubitong Recipe in the Treatment of Osteoarthritis WB Human 1 : 1000 /SW1353 cells

LncRNA TUG1 Promotes Apoptosis, Invasion, and Angiogenesis of Retinal Endothelial Cells in Retinopathy of Prematurity via MiR-145-5p. Frontiers in Medicine2022 Apr 4;9:803214. Mouse 1:200 retinal tissue

HIF-1α Regulates Osteogenesis of Periosteum-Derived Stem Cells Under Hypoxia Conditions via Modulating POSTN Expression Front Cell Dev Biol. 2022 Feb 17;10:836285. IF Mouse 1:200

Genetically engineered magnetic nanocages for cancer magneto-catalytic theranostics. Nature Communications Nat Commun. 2020 Oct;11(1):1-11 WB Human A549 cell

Deciphering the performance of polo-like kinase 1 in triple-negative breast cancer progression according to the centromere protein U-phosphorylation pathway. American Journal of Cancer Research Am J Cancer Res. 2021; 11(5): 2142–2158 WB Human MDA-MB-231cell， CAL-51 cancer cell

A COX-2/sEH dual inhibitor PTUPB ameliorates cecal ligation and puncture-induced sepsis in mice via anti-inflammation and anti-oxidative stress. BIOMEDICINE & PHARMACOTHERAPY Biomed Pharmacother. 2020 Jun;126:109907 WB Mouse 1:2000 liver, lung, , kidney

HIF-1α Mediates Osteoclast-Induced Mandibular Condyle Growth via AMPK Signaling:. JOURNAL OF DENTAL RESEARCH 2020 Jun 29 WB Mouse 1:300 bone marrow monocyte (BMM)

Enhancement of bone regeneration with the accordion technique via HIF‐1α/VEGF activation in a rat distraction osteogenesis model. Journal of Tissue Engineering and Regenerative Medicine 2017 Nov 17 WB Rat bone

Heparanase from triple‑negative breast cancer and platelets acts as an enhancer of metastasis. INTERNATIONAL JOURNAL OF ONCOLOGY 2020 Aug 27 WB Human 1 : 2000 MDA-MB-231 cell

Adiponectin alleviates the symptoms of ischemic renal disease by inhibiting renal cell apoptosis. LIFE SCIENCES Life Sci. 2021 Jan;265:118825 IHC Mouse 1:200 Kidney

Differential susceptibility of PC12 and BRL cells and the regulatory role of HIF-1α signaling pathway in response to acute methylmercury exposure under normoxia. TOXICOLOGY LETTERS Toxicol Lett. 2020 Oct;331:82 WB Rat PC12 cell,BRL cell

Partial deficiency of HIF-1α in chondrocytes effected bone repair of mandibular condylar neck. ARCHIVES OF ORAL BIOLOGY Arch Oral Biol. 2021 Feb;122:105023 IHC Mouse 1:200 Chondrocytes

Sprayed copper peroxide nanodots for accelerating wound healing in a multidrug-resistant bacteria infected diabetic ulcer. Nanoscale Nanoscale. 2021 Oct;13(37):15937-15951 WB Human HUVECs

PDGF-mediated PI3K/AKT/β-catenin signaling regulates gap junctions in corpus cavernosum smooth muscle cells. EXPERIMENTAL CELL RESEARCH Exp Cell Res. 2018 Jan;362:252 WB Rat 1:500 corpus cavernosum smooth muscle cells (CCSMCs)

CD44-Targeting Oxygen Self-Sufficient Nanoparticles for Enhanced Photodynamic Therapy Against Malignant Melanoma. International Journal of Nanomedicine Int J Nanomed. 2020; 15: 10401–10416 IHC，IF Mouse MV3 cell-Xenograft

Grade-targeted nanoparticles for improved hypoxic tumor microenvironment and enhanced photodynamic cancer therapy. Nanomedicine 2021 Feb 03 IF Mouse MDA-MB-231-Xenograft

Isorhamnetin Promotes MKN-45 Gastric Cancer Cell Apoptosis by Inhibiting PI3K-Mediated Adaptive Autophagy in a Hypoxic Environment. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY J Agr Food Chem. 2021;XXXX(XXX):XXX-XXX WB，IF Human MKN-45 cells

HIF‐1α facilitates osteocyte‐mediated osteoclastogenesis by activating JAK2/STAT3 pathway in vitro. JOURNAL OF CELLULAR PHYSIOLOGY 2019 Apr 29 IF Mouse 1:200 MLO-Y4 cell

Mandibular osteotomy‐induced hypoxia enhances osteoclast activation and acid secretion by increasing glycolysis. JOURNAL OF CELLULAR PHYSIOLOGY J Cell Physiol. 2019 Jul;234(7):11165-11175 IHC,IF Rabbit,Mouse 1:200,1:200 mandibular tissue RAW264.7 cell

Dissecting the roles of Ephrin-A3 in malignant peripheral nerve sheath tumor by TALENs. ONCOLOGY REPORTS Oncol Rep. 2015 Jul;34(1):391-398 WB Human ST88-14 cell, sNF96.2 cell

Total Flavonoids from Carya cathayensis Sarg. Leaves Alleviate H9c2 Cells Hypoxia/Reoxygenation Injury via Effects on miR-21 Expression, PTEN/Akt, and the Bcl-2/Bax Pathway. Evidence-based Complementary and Alternative Medicine Evid-Based Compl Alt. 2018;2018:8617314 WB Rat 1:1000 H9c2 cell

Down-regulation of FoxM1 by thiostrepton or small interfering RNA inhibits proliferation, transformation ability and angiogenesis, and induces apoptosis of nasopharyngeal carcinoma cells. International Journal of Clinical and Experimental Pathology Int J Clin Exp Patho. 2014; 7(9): 5450–5460 WB Human Nasopharyngeal carcinoma (NPC) cell

CD90 affects the biological behavior and energy metabolism level of gastric cancer cells by targeting the PI3K/AKT/HIF‑1α signaling pathway. Oncology Letters Oncol Lett. 2021 Mar;21(3):1-1 WB Human 1:1000 AGS cell

Cynaroside prevents macrophage polarization into pro-inflammatory phenotype and alleviates cecal ligation and puncture-induced liver injury by targeting PKM2/HIF-1α axis. FITOTERAPIA Fitoterapia. 2021 Jul;152:104922 WB Mouse Liver

Hypoxia promotes 786-O cells invasiveness and resistance to sorafenib via HIF-2α/COX-2. MEDICAL ONCOLOGY Med Oncol. 2015 Jan;32(1):1-9 WB Human 786-O cell

Nickel-Smelting Fumes Increased the Expression of HIF-1α through PI3K/ERK Pathway in NIH/3T3 Cells. JOURNAL OF OCCUPATIONAL HEALTH 2016 Aug 04 IHC Rat lung

High-purity magnesium pin enhances bone consolidation in distraction osteogenesis model through activation of the VHL/HIF-1α/VEGF signaling:. JOURNAL OF BIOMATERIALS APPLICATIONS J Biomater Appl. 2020;35(2):224-236 IHC Rat femurs

Junsheng, Mu, et al. "Myocardial Patch Formation by Three-Dimensional Culture of Bone Marrow Mesenchymal Stem Cells with 3-Hydroxybutyrate-Co-4-Hydroxybutyrate Under Hypoxia." Journal of Biomaterials and Tissue Engineering 10.7 (2020): 922-929.

“Deciphering the performance of polo-like kinase 1 in triple-negative breast cancer progression according to the centromere protein U-phosphorylation pathway.” American journal of cancer research vol. 11,5 2142-2158. 15 May. 2021

Adiponectin alleviates the symptoms of ischemic renal disease by inhibiting renal cell apoptosis. LIFE SCIENCES Life Sci. 2021 Jan;265:118825 IHC Mouse 1:200 Kidney

Deciphering the performance of polo-like kinase 1 in triple-negative breast cancer progression according to the centromere protein U-phosphorylation pathway. American Journal of Cancer Research Am J Cancer Res. 2021; 11(5): 2142–2158 WB Human MDA-MB-231cell， CAL-51 cancer cell

Losartan protects against intermittent hypoxia-induced peritubular capillary loss by modulating the renal renin–angiotensin system and angiogenesis factors. ACTA BIOCHIMICA ET BIOPHYSICA SINICA Acta Bioch Bioph Sin. 2020 Jan;52(1):38-48 WB Rat 1:300 Kidney cortex

Small extracellular vesicles derived from hypoxic mesenchymal stem cells promote vascularized bone regeneration through the miR-210-3p/EFNA3/PI3K pathway Acta Biomaterialia Jiawen Si WB Rat

Kaempferol prevents angiogenesis of rat intestinal microvascular endothelial cells induced by LPS and TNF-α via inhibiting VEGF/Akt/p38 signaling pathways and maintaining gut-vascular barrier integrity CHEMICO-BIOLOGICAL INTERACTIONS Zhongjie Liu WB Rat

Nanocarrier of Pin1 inhibitor based on supercritical fluid technology inhibits cancer metastasis by blocking multiple signaling pathways Regenerative Biomaterials Dayun Yang WB,IHC Human,Mouse lung tissues HuH7 cell,HepG2 cell

Decreasing expression of HIF-1α, VEGF-A, and Ki67 with efficacy of neoadjuvant therapy in locally advanced cervical cancer. Tingkuan Zhao IHC Human 1:200 locally advanced cervical cancer (LACC) tissue,normal cervical tissue

Mailuoning oral liquid attenuates convalescent cerebral ischemia by inhibiting AMPK/mTOR-associated apoptosis and promoting CREB/BDNF-mediated neuroprotection. Ninghua Tan WB Rat cortex tissue PC-12 cells

Loss of NDUFS1 promotes gastric cancer progression by activating the mitochondrial ROS-HIF1α-FBLN5 signaling pathway. BRITISH JOURNAL OF CANCER Jin Zhou WB Human 1:1000 MKN45 cell,N87 cell

A bone-targeting near-infrared luminescence nanocarrier facilitates alpha-ketoglutarate efficacy enhancement for osteoporosis therapy Acta Biomaterialia Chunan Cheng IF,WB Mouse 1:200,1:1000 RAW264.7 cell,MC3T3-E1 cell

Targeting HIF-1α alleviates the inflammatory responses and rebuilds the CD4+ T cell subsets balance in the experimental autoimmune myasthenia gravis inflammation model via regulating cellular and humoral immunity LIFE SCIENCES Lu Yu WB Rat 1:1000 lymphocytes

Biomimetic Hydrogel Containing Copper Sulfide Nanoparticles and Deferoxamine for Photothermal Therapy of Infected Diabetic Wounds Advanced Healthcare Materials Haijun Shen IHC Mouse 1:100 skin tissue

Hypoxia Aggravates Neuron Ferroptosis in Early Brain Injury Following Subarachnoid Hemorrhage via NCOA4-Meditated Ferritinophagy Antioxidants Zixuan Yuan WB,IF Mouse 1:1000,1:200 brain tissue HT22 cell

Manganese overexposure results in ferroptosis through the HIF-1α/p53/SLC7A11 pathway in ICR mouse brain and PC12 cells ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Jian Chen WB Rat PC12 cell

Microneedle Patch Loaded with Calcium Peroxide Nanoparticles for Oxygen Healing and Biofilm Inhibition in Diabetic Wound Healing ACS Applied Nano Materials Haijun Shen WB,IHC Mouse,Human skin tissue Human umbilical vein endothelial cell(HUVEC)

Topical frankincense treatment for frostbite based on microcirculation improvements JOURNAL OF ETHNOPHARMACOLOGY Zhao Zhang IHC Rat skin tissue

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