• Target：
• Histone H1

• Gene Name：
• HIST1H1B

• Protein Name：
• Histone H1.5

• Human Gene Id：
• 3009/3007/3008

• Human Swiss Prot No：
• P16401/P16402/P10412

• Mouse Gene Id：
• 56702/14957/50709

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human Histone H1. AA range:1-50

• Specificity：
• Histone H1 Polyclonal Antibody detects endogenous levels of Histone H1 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• HIST1H1B;H1F5;Histone H1.5;Histone H1a;Histone H1b;Histone H1s-3;HIST1H1D;H1F3;Histone H1.3;Histone H1c;Histone H1s-2;HIST1H1E;H1F4;Histone H1.4;Histone H1b;Histone H1s-4

• Observed Band(KD)：
• 31 28kD

• Background：
• Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],

• Function：
• function:Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.,similarity:Belongs to the histone H1/H5 family.,

• Subcellular Location：
• Nucleus. Chromosome. According to PubMed:15911621 more commonly found in heterochromatin. According to PubMed:10997781 associates with actively transcribed chromatin and not heterochromatin.

• Expression：
• Ubiquitous. Expressed in the majority of the cell lines tested and in testis.

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