HPK1 Polyclonal Antibody
- Catalog No.:YT2225
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse
- Target:
- HPK1
- Fields:
- >>MAPK signaling pathway
- Gene Name:
- MAP4K1
- Protein Name:
- Mitogen-activated protein kinase kinase kinase kinase 1
- Human Gene Id:
- 11184
- Human Swiss Prot No:
- Q92918
- Mouse Gene Id:
- 26411
- Mouse Swiss Prot No:
- P70218
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human MEKKK 1. AA range:371-420
- Specificity:
- HPK1 Polyclonal Antibody detects endogenous levels of HPK1 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. ELISA: 1:10000.. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- MAP4K1;HPK1;Mitogen-activated protein kinase kinase kinase kinase 1;Hematopoietic progenitor kinase;MAPK/ERK kinase kinase kinase 1;MEK kinase kinase 1;MEKKK 1
- Observed Band(KD):
- 91kD
- Background:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.,similarity:Contains 1 CNH domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with MAP3K1.,tissue specificity:Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.,
- Function:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.,similarity:Contains 1 CNH domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with MAP3K1.,tissue specificity:Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.,
- Subcellular Location:
- intracellular,cytoplasm,membrane,
- Expression:
- Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.
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- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using HPK1 Polyclonal Antibody diluted at 1:1000
- Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Western blot analysis of lysates from HepG2 cells, using MEKKK 1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HUVECcells using MEKKK 1 antibody.