HSF2 Polyclonal Antibody
- Catalog No.:YT2237
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- HSF2
- Gene Name:
- HSF2
- Protein Name:
- Heat shock factor protein 2
- Human Gene Id:
- 3298
- Human Swiss Prot No:
- Q03933
- Mouse Gene Id:
- 15500
- Mouse Swiss Prot No:
- P38533
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human HSF2. AA range:401-450
- Specificity:
- HSF2 Polyclonal Antibody detects endogenous levels of HSF2 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- HSF2;HSTF2;Heat shock factor protein 2;HSF 2;Heat shock transcription factor 2;HSTF 2
- Observed Band(KD):
- 85kD
- Background:
- heat shock transcription factor 2(HSF2) Homo sapiens The protein encoded by this gene belongs to the HSF family of transcription factors that bind specifically to the heat-shock promoter element and activate transcription. Heat shock transcription factors activate heat-shock response genes under conditions of heat or other stresses. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011],
- Function:
- function:DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.,similarity:Belongs to the HSF family.,subcellular location:Cytoplasmic during normal growth and moves to the nucleus upon activation.,subunit:DNA-binding homotrimer in stressed or heat shocked cells, otherwise found as a homodimer.,
- Subcellular Location:
- Cytoplasm . Nucleus . Cytoplasmic during normal growth and moves to the nucleus upon activation.
- Expression:
- Bladder,Brain,PCR rescued clones,Testis,
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- July 13-2018
- Antibody-FAQs
- Products Images
- Western blot analysis of lysates from LOVO and A549 cells, using HSF2 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HeLa cells using HSF2 antibody.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).