HXK I Polyclonal Antibody

    • Catalog No.：YT2265
    • Applications：WB;IHC;IF;ELISA
    • Reactivity：Human;Mouse;Rat
      • Target：
      • HXK I
      • Fields：
      • >>Glycolysis / Gluconeogenesis;>>Fructose and mannose metabolism;>>Galactose metabolism;>>Starch and sucrose metabolism;>>Amino sugar and nucleotide sugar metabolism;>>Neomycin, kanamycin and gentamicin biosynthesis;>>Metabolic pathways;>>Carbon metabolism;>>Biosynthesis of nucleotide sugars;>>HIF-1 signaling pathway;>>Insulin signaling pathway;>>Type II diabetes mellitus;>>Carbohydrate digestion and absorption;>>Shigellosis;>>Central carbon metabolism in cancer
      • Gene Name：
      • HK1
      • Protein Name：
      • Hexokinase-1
      • Human Gene Id：
      • 3098
      • Human Swiss Prot No：
      • P19367
      • Mouse Swiss Prot No：
      • P17710
      • Immunogen：
      • The antiserum was produced against synthesized peptide derived from human HXK1. AA range:31-80
      • Specificity：
      • HXK I Polyclonal Antibody detects endogenous levels of HXK I protein.
      • Formulation：
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source：
      • Polyclonal, Rabbit,IgG
      • Dilution：
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
      • Purification：
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration：
      • 1 mg/ml
      • Storage Stability：
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name：
      • HK1;Hexokinase-1;Brain form hexokinase;Hexokinase type I;HK I
      • Observed Band(KD)：
      • 109kD
      • Background：
      • Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific. [provided by RefSeq, Apr 2016],
      • Function：
      • catalytic activity:ATP + D-hexose = ADP + D-hexose 6-phosphate.,disease:Defects in HK1 are the cause of hexokinase deficiency [MIM:235700]. Hexokinase deficiency is a rare autosomal recessive disease with nonspherocytic hemolytic anemia as the predominant clinical feature.,domain:The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.,enzyme regulation:Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.,miscellaneous:In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).,online information:Hexokinase entry,pathway:Carbohydrate metabolism; hexose metabolism.,similarity:Belongs to the hexokinase family.,subcellular location:Its hydrophobic N-ter
      • Subcellular Location：
      • Mitochondrion outer membrane ; Peripheral membrane protein . Cytoplasm, cytosol . The mitochondrial-binding peptide (MBP) region promotes association with the mitochondrial outer membrane (Probable). Dissociates from the mitochondrial outer membrane following inhibition by N-acetyl-D-glucosamine, leading to relocation to the cytosol (PubMed:27374331). .
      • Expression：
      • Isoform 2: Erythrocyte specific (Ref.6). Isoform 3: Testis-specific (PubMed:10978502). Isoform 4: Testis-specific (PubMed:10978502).
      • Products Images
      • Gao, Yunshu, et al. "Overexpression of metabolic markers HK1 and PKM2 contributes to lymphatic metastasis and adverse prognosis in Chinese gastric cancer." International journal of clinical and experimental pathology 8.8 (2015): 9264.
      • Western blot analysis of HXK I Polyclonal Antibody, using Hela, MCF7,SW480,293T cell, 4° over night, secondary antibody(cat: RS0002 was diluted at 1:10000, 37° 1hour.
      • Western Blot analysis of various cells using HXK I Polyclonal Antibody diluted at 1:1000
      • Western Blot analysis of HeLa cells using HXK I Polyclonal Antibody diluted at 1:1000
      • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Western blot analysis of lysates from HeLa cells, using HXK1 Antibody. The lane on the right is blocked with the synthesized peptide.

      Attributes

      • Code：
      • YT2265
      • Size
      • 40μL / 100μL / 200μL
      • Price$：
      • 150 / 280 / 450
      • Delivery：
      • 2024-04-18
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