• Target：
• LARG

• Fields：
• >>Vascular smooth muscle contraction;>>Axon guidance;>>Platelet activation;>>C-type lectin receptor signaling pathway;>>Regulation of actin cytoskeleton;>>Pathogenic Escherichia coli infection;>>Yersinia infection;>>Tuberculosis;>>Human cytomegalovirus infection;>>Pathways in cancer;>>Proteoglycans in cancer

• Gene Name：
• ARHGEF12

• Protein Name：
• Rho guanine nucleotide exchange factor 12

• Human Gene Id：
• 23365

• Human Swiss Prot No：
• Q9NZN5

• Mouse Swiss Prot No：
• Q8R4H2

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human ARHGEF12. AA range:449-498

• Specificity：
• LARG Polyclonal Antibody detects endogenous levels of LARG protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000;IHC 1:50-300

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• ARHGEF12;KIAA0382;LARG;Rho guanine nucleotide exchange factor 12;Leukemia-associated RhoGEF

• Observed Band(KD)：
• 173kD

• Background：
• Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014],

• Function：
• disease:A chromosomal aberration involving ARHGEF12 may be a cause of acute leukemia. Translocation t(11;11)(q23;23) with MLL.,function:May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 RGSL (RGS-like) domain.,subcellular location:Translocated to the membrane upon stimulation.,subunit:Interacts with GNA12 and GNA13, probably through the RGS-like domain. Interacts with RHOA, PLXNB1 and PLXNB2. Interacts through its PDZ domain

• Subcellular Location：
• Cytoplasm . Membrane . Translocated to the membrane upon stimulation. .

• Expression：
• Ubiquitously expressed. Isoform 2 is found in jejunum and testis.

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