N/H/K-Ras Polyclonal Antibody
- Catalog No.:YT2960
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- N/H/K-Ras
- Fields:
- >>EGFR tyrosine kinase inhibitor resistance;>>Endocrine resistance;>>MAPK signaling pathway;>>ErbB signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>Chemokine signaling pathway;>>FoxO signaling pathway;>>Sphingolipid signaling pathway;>>Phospholipase D signaling pathway;>>Mitophagy - animal;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>Apoptosis;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Cellular senescence;>>Axon guidance;>>VEGF signaling pathway;>>Apelin signaling pathway;>>Gap junction;>>Signaling pathways regulating pluripotency of stem cells;>>C-type lectin receptor signaling pathway;>>Natural killer cell mediated cytotoxicity;>>T cell receptor signaling pathway;>>B cell receptor signaling pathway;>>Fc epsilon RI signaling pathway;>>Thermogenesis;>>Long-term potentiation;>>Neurotrophin signaling pathway;>>Cholinergic synapse;>>Serotonergic synapse;>>Long-term depression;>>Regulation of actin
- Gene Name:
- NRAS/HRAS/KRAS
- Protein Name:
- GTPase Nras/GTPase Hras/GTPase Kras
- Human Gene Id:
- 3265/3845/4893
- Human Swiss Prot No:
- P01111/P01112/P01116
- Mouse Gene Id:
- 15461/16653
- Rat Gene Id:
- 24605/293621/24525
- Rat Swiss Prot No:
- Q04970/P20171/P08644
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human RASH/RASK. AA range:1-50
- Specificity:
- N/H/K-Ras Polyclonal Antibody detects endogenous levels of N/H/K-Ras protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000. IF 1:100-300 Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- NRAS;HRAS1;GTPase NRas;Transforming protein N-Ras;HRAS;HRAS1;GTPase HRas;H-Ras-1;Ha-Ras;Transforming protein p21;c-H-ras;p21ras;KRAS;KRAS2;RASK2;GTPase KRas;K-Ras 2;Ki-Ras;c-K-ras;c-Ki-ras
- Observed Band(KD):
- 21kD
- Background:
- This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011],
- Function:
- disease:Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia.,disease:Mutations which change AA 12, 13 or 61 activate the potential of Ras to transform cultured cells and are implicated in a variety of human tumors.,enzyme regulation:Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).,function:Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.,online information:NRAS mutation db,online information:RAS proteins entry,PTM:Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membran
- Subcellular Location:
- Cell membrane ; Lipid-anchor ; Cytoplasmic side . Golgi apparatus membrane ; Lipid-anchor . Shuttles between the plasma membrane and the Golgi apparatus. .
- Expression:
- Bone marrow,Bone-marrow,Brain,Fibrosarcoma,Kidney,Leukemia,Lung car
Mst3b Promotes Spinal Cord Neuronal Regeneration by Promoting Growth Cone Branching Out in Spinal Cord Injury Rats. MOLECULAR NEUROBIOLOGY 2014 Jul 03 WB Rat 1:1000 PC12 cell
CPNE1 silencing inhibits the proliferation, invasion and migration of human osteosarcoma cells. ONCOLOGY REPORTS 2017 Dec 04 WB Human 1:1000 Saos-2 cell
MAPK pathway mutations in head and neck cancer affect immune microenvironments and ErbB3 signaling. Life Science Alliance 2020 Jun;3(6):e201900545 WB Human HSC-4 cell,HSC-6 cell
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- May 20-2022
- Cell-Based ELISA│解您多样本WB检测之困扰
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Immunofluorescence analysis of Siha cell. 1,primary Antibody was diluted at 1:100(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - AFluor 488 Secondary antibody (catalog No:RS3211) was diluted at 1:500(room temperature, 50min).
- Immunofluorescence analysis of Siha cell. 1,primary Antibody was diluted at 1:100(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - AFluor 594 Secondary antibody(catalog No: RS3611) was diluted at 1:500(room temperature, 50min).
- Western Blot analysis of various cells using N/H/K-Ras Polyclonal Antibody diluted at 1:1000
- Western Blot analysis of 293 cells using N/H/K-Ras Polyclonal Antibody diluted at 1:1000
- Immunohistochemistry analysis of paraffin-embedded human brain tissue, using RASH/RASK Antibody. The picture on the right is blocked with the synthesized peptide.