Neuro D2 Polyclonal Antibody

    • Catalog No.:YT3062
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • Neuro D2
      • Gene Name:
      • NEUROD2
      • Protein Name:
      • Neurogenic differentiation factor 2
      • Human Gene Id:
      • 4761
      • Human Swiss Prot No:
      • Q15784
      • Mouse Swiss Prot No:
      • Q62414
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human NDF2. AA range:61-110
      • Specificity:
      • Neuro D2 Polyclonal Antibody detects endogenous levels of Neuro D2 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • NEUROD2;BHLHA1;NDRF;Neurogenic differentiation factor 2;NeuroD2;Class A basic helix-loop-helix protein 1;bHLHa1;NeuroD-related factor;NDRF
      • Observed Band(KD):
      • 48kD
      • Background:
      • This gene encodes a member of the neuroD family of neurogenic basic helix-loop-helix (bHLH) proteins. Expression of this gene can induce transcription from neuron-specific promoters, such as the GAP-43 promoter, which contain a specific DNA sequence known as an E-box. The product of the human gene can induce neurogenic differentiation in non-neuronal cells in Xenopus embryos, and is thought to play a role in the determination and maintenance of neuronal cell fates. [provided by RefSeq, Jul 2008],
      • Function:
      • function:Appears to mediate neuronal differentiation.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein.,
      • Subcellular Location:
      • Nucleus .
      • Expression:
      • Brain,
      • Products Images
      • Western Blot analysis of various cells using Neuro D2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • Western blot analysis of lysates from 293, COLO, and Jurkat cells, using NDF2 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western blot analysis of the lysates from COLO205 cells using NDF2 antibody.
      • Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).