NFκB-p100 Polyclonal Antibody

    • Catalog No.:YT3093
    • Applications:WB;IHC;IP;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • NF-κB p100/p52
      • Fields:
      • >>MAPK signaling pathway;>>NF-kappa B signaling pathway;>>Osteoclast differentiation;>>C-type lectin receptor signaling pathway;>>Legionellosis;>>Human T-cell leukemia virus 1 infection;>>Epstein-Barr virus infection;>>Pathways in cancer;>>Viral carcinogenesis;>>Breast cancer
      • Gene Name:
      • NFKB2
      • Protein Name:
      • Nuclear factor NF-kappa-B p100 subunit
      • Human Gene Id:
      • 4791
      • Human Swiss Prot No:
      • Q00653
      • Mouse Swiss Prot No:
      • Q9WTK5
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human NF-kappaB p100/p52. AA range:833-882
      • Specificity:
      • NFκB-p100 Polyclonal Antibody detects endogenous levels of NFκB-p100 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. Immunoprecipitation: 2-5 ug:mg lysate. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • NFKB2;LYT10;Nuclear factor NF-kappa-B p100 subunit;DNA-binding factor KBF2;H2TF1;Lymphocyte translocation chromosome 10 protein;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2;Oncogene Lyt-10;Lyt10
      • Molecular Weight(Da):
      • 97kD
      • Background:
      • nuclear factor kappa B subunit 2(NFKB2) Homo sapiens This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013],
      • Function:
      • disease:A chromosomal aberration involving NFKB2 is found in a case of B-cell non Hodgkin lymphoma (B-NHL). Translocation t(10;14)(q24;q32) with IGHA1. The resulting oncogene is also called Lyt-10C alpha variant.,disease:A chromosomal aberration involving NFKB2 is found in a cutaneous T-cell leukemia (C-TCL) cell line. This rearrangement produces the p80HT gene which encodes for a truncated 80 kDa protein (p80HT).,disease:In B-cell leukemia (B-CLL) cell line, LB40 and EB308, can be found after heterogeneous chromosomal aberrations, such as internal deletions.,domain:The C-terminus of p100 might be involved in cytoplasmic retention, inhibition of DNA-binding by p52 homodimers, and/or transcription activation.,domain:The glycine-rich region (GRR) appears to be a critical element in the generation of p52.,function:NF-kappa-B is a pleiotropic transcription factor which is present in almost a
      • Subcellular Location:
      • Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).
      • Expression:
      • Leukemia,Lymph,Thymus,
      • Products Images
      • Western blot analysis of lysates from RAW264.7 cells treated with EGF 200ng/ml 30', using NF-kappaB p100 Antibody. Primary Antibody was diluted at 1:1000 4° over night,secondary antibody(Immunoway cat:RS23920)was diluted at 1:10000, 37° 1hour.
      • Immunofluorescence analysis of Hela cell. 1,NFκB-p100 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). ATG5 mouse Monoclonal Antibody(3C7)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
      • Western blot analysis of KB K562 Hela 293T lysis using NFκB-p100 antibody. Antibody was diluted at 1:1000
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using NF-kappaB p100/p52 Antibody. The picture on the right is blocked with the synthesized peptide.