• Target：
• NFkB p65

• Fields：
• >>Antifolate resistance;>>MAPK signaling pathway;>>Ras signaling pathway;>>cAMP signaling pathway;>>Chemokine signaling pathway;>>NF-kappa B signaling pathway;>>HIF-1 signaling pathway;>>Sphingolipid signaling pathway;>>Mitophagy - animal;>>PI3K-Akt signaling pathway;>>Apoptosis;>>Longevity regulating pathway;>>Cellular senescence;>>Osteoclast differentiation;>>Neutrophil extracellular trap formation;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>RIG-I-like receptor signaling pathway;>>Cytosolic DNA-sensing pathway;>>C-type lectin receptor signaling pathway;>>IL-17 signaling pathway;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>T cell receptor signaling pathway;>>B cell receptor signaling pathway;>>TNF signaling pathway;>>Neurotrophin signaling pathway;>>Prolactin signaling pathway;>>Adipocytokine signaling pathway;>>Relaxin signaling pathway;>>Insulin resistance;>>Non-alcoholic fatty liver disease;>>AGE-RAGE signaling pathway in diabe

• Gene Name：
• RELA

• Protein Name：
• Transcription factor p65

• Human Gene Id：
• 5970

• Human Swiss Prot No：
• Q04206

• Mouse Gene Id：
• 19697

• Mouse Swiss Prot No：
• Q04207

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human NF-kappaB p65. AA range:247-296

• Specificity：
• NFκB-p65 Polyclonal Antibody detects endogenous levels of NFκB-p65 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• RELA;NFKB3;Transcription factor p65;Nuclear factor NF-kappa-B p65 subunit;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

• Observed Band(KD)：
• 60kD

• Background：
• NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],

• Function：
• function:NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by in

• Subcellular Location：
• Nucleus . Cytoplasm . Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B) (PubMed:1493333). Colocalized with DDX1 in the nucleus upon TNF-alpha induction (PubMed:19058135). Colocalizes with GFI1 in the nucleus after LPS stimulation (PubMed:20547752). Translocation to the nucleus is impaired in L.monocytogenes infection (PubMed:20855622). .

• Expression：
• Bone,Colon,Pancreas,Placenta,

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LXA4 ameliorates cerebrovascular endothelial dysfunction by reducing acute inflammation after subarachnoid hemorrhage in rats. NEUROSCIENCE Neuroscience. 2019 Jun;408:105 WB Rat 1:1000 brain

Fra-1 Inhibits Cell Growth and the Warburg Effect in Cervical Cancer Cells via STAT1 Regulation of the p53 Signaling Pathway. Frontiers in Cell and Developmental Biology Front Cell Dev Biol. 2020 Sep;0:1022 WB Human 1 : 1000 HeLa cell

Allyl Sulfide Counteracts 1-Bromopropane-Induced Neurotoxicity by Inhibiting Neuroinflammation and Oxidative Stress. TOXICOLOGICAL SCIENCES 2019 Feb 01 WB Rat cortex

The antitumor effect of hinesol, extract from Atractylodes lancea (Thunb.) DC. by proliferation, inhibition, and apoptosis induction via MEK/ERK and NF‐κB pathway in non–small cell lung cancer cell lines A549 and NCI‐H1299. JOURNAL OF CELLULAR BIOCHEMISTRY 2019 Jul 24 WB Human A549 cell

Exogenous ghrelin ameliorates acute lung injury by modulating the nuclear factor κB inhibitor kinase/nuclear factor κB inhibitor/nuclear factor κB pathway after hemorrhagic shock. INTERNATIONAL IMMUNOPHARMACOLOGY Int Immunopharmacol. 2019 Apr;69:95 WB Rat 1:1000 lung tissue

The receptor for activated protein kinase C promotes cell growth, invasion and migration in cervical cancer. INTERNATIONAL JOURNAL OF ONCOLOGY Int J Oncol. 2017 Nov;51(5):1497-1507 WB Human 1:1000 cervical cancer tissues CaSki cell

The Antitumor Effect of Suicide Gene Therapy Using Bifidobacterium infantis–mediated Herpes Simplex Virus Thymidine Kinase/Ganciclovir in a Nude Mice Model of Renal Cell Carcinoma. UROLOGY 2014 Aug 12 WB Mouse 1:500 786-O cell-Xenograft

Chronic dexamethasone treatment results in hippocampal neurons injury due to activate NLRP1 inflammasome in vitro. INTERNATIONAL IMMUNOPHARMACOLOGY Int Immunopharmacol. 2017 Aug;49:222 WB Rat hippocampus neurons

p21-activated kinase 4 as a switch between caspase-8 apoptosis and NF-κB survival signals in response to TNF-α in hepatocarcinoma cells. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2018 Aug 24 WB Human SK-Hep1 cell, HepG2 cell

Dopamine receptor D2 inhibition alleviates diabetic hepatic stellate cells fibrosis by regulating the TGF‐β1/Smads and NFκB pathways. CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY Clin Exp Pharmacol P. 2021 Mar;48(3):370-380 WB Rat 1 : 1000 Liver hepatic stellate cells (HSCs)

Guiqi Baizhu Decoction Alleviates Radiation Inflammation in Rats by Modulating the Composition of the Gut Microbiota. Evidence-based Complementary and Alternative Medicine 2020 Oct 20 WB Rat Colon

Safety profile of the transcription factor EB (TFEB)-based gene therapy through intracranial injection in mice. Translational Neuroscience Transl Neurosci. 2020 Jan;11(1):241-250 WB Mouse brain tissue

Lu, Qian, et al. "Norisoboldine suppresses VEGF-induced endothelial cell migration via the cAMP-PKA-NF-κB/Notch1 pathway." PloS one 8.12 (2013): e81220.

The receptor for activated protein kinase C promotes cell growth, invasion and migration in cervical cancer. INTERNATIONAL JOURNAL OF ONCOLOGY Int J Oncol. 2017 Nov;51(5):1497-1507 WB Human 1:1000 cervical cancer tissues CaSki cell

Correlation of In Vivo and In Vitro Assay Results for Assessment of Free Radical Scavenging Activity of Green Tea Nutraceuticals. JOURNAL OF FOOD SCIENCE J Food Sci. 2016 Jul;81(7):C1707-C1715 ELISA Rat hepatic

LXA4 ameliorates cerebrovascular endothelial dysfunction by reducing acute inflammation after subarachnoid hemorrhage in rats. NEUROSCIENCE Neuroscience. 2019 Jun;408:105 WB Rat 1:1000 brain

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Hydrogen gas ameliorates acute alcoholic liver injury via anti-inflammatory and antioxidant effects and regulation of intestinal microbiota. Weiping Fan WB Mouse 1:1000 liver tissue

Hypoxic preconditioned mesenchymal stem cells ameliorate rat brain injury after cardiopulmonary resuscitation by suppressing neuronal pyroptosis. Feng Chen WB Rat cortical tissue

Dangshen Huangjiu exerts prevention of gastric mucosal injury and inhibits Akt/NF-κB signaling. Fangdi Hu WB Rat,Human gastric mucosal tissues GES-1 cell

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