OPN Polyclonal Antibody
- Catalog No.:YT3467
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- OPN
- Fields:
- >>PI3K-Akt signaling pathway;>>Apelin signaling pathway;>>Focal adhesion;>>ECM-receptor interaction;>>Toll-like receptor signaling pathway;>>GnRH secretion;>>Human papillomavirus infection
- Gene Name:
- SPP1
- Protein Name:
- Osteopontin
- Human Gene Id:
- 6696
- Human Swiss Prot No:
- P10451
- Mouse Gene Id:
- 20750
- Mouse Swiss Prot No:
- P10923
- Rat Gene Id:
- 25353
- Rat Swiss Prot No:
- P08721
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human Osteopontin. AA range:261-310
- Specificity:
- OPN Polyclonal Antibody detects endogenous levels of OPN protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500-2000;IHC 1:100-500;IF ICC 1:100-500;ELISA 1:5000-20000
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- SPP1;BNSP;OPN;PSEC0156;Osteopontin;Bone sialoprotein 1;Nephropontin;Secreted phosphoprotein 1;SPP-1;Urinary stone protein;Uropontin
- Observed Band(KD):
- 60kD
- Background:
- secreted phosphoprotein 1(SPP1) Homo sapiens The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011],
- Function:
- disease:This protein plays a principal role in urinary stone formation as the stone matrix.,function:Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.,function:Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.,online information:Osteopontin entry,PTM:Extensively phosphorylated on clustered serine residues.,PTM:N- and O-glycosylated.,similarity:Belongs to the osteopontin family.,subunit:Ligand for integrin alpha-V/beta-3.,
- Subcellular Location:
- Secreted.
- Expression:
- Bone. Found in plasma.
CD137-CD137L Aggravates Calcification of Vascular Smooth Muscle Cell and Vasculature of ApoE−/− Mice Via Rab7-Mediated Autophagy IF Mouse 1:200 /VSMCs
In vitro effects of recombinant adenovirus-mediated bone morphogenetic protein 2/vascular endothelial growth factor 165 on osteogenic differentiation of bone marrow mesenchymal stem cells. Artificial Cells Nanomedicine and Biotechnology 2016 Jan 13 WB Mouse 1:500 C3H10T1/2 cell
Oral Hydrogen-Rich Water Alleviates Oxalate-Induced Kidney Injury by Suppressing Oxidative Stress, Inflammation, and Fibrosis. Frontiers in Medicine Front Med-Lausanne. 2021; 8: 713536 IHC Mouse 1:100 Kidney
Elevated c-fos expression is correlated with phenotypic switching of human vascular smooth muscle cells derived from lower limb venous varicosities. Journal of Vascular Surgery-Venous and Lymphatic Disorders J Vasc Surg-Venous L. 2021 Jan;9:242 WB Human 1:1000 VSMCs
4D Printing of Personalized-Tunable Biomimetic Periosteum with Anisotropic Microstructure for Accelerated Vascularization and Bone Healing. Mengfei Yu WB Human human mesenchymal stem cells (hMSCs)
Increased abundance of bacteria of the family Muribaculaceae achieved by fecal microbiome transplantation correlates with the inhibition of kidney calcium oxalate stone deposition in experimental rats. Pan TieJun IHC Rat kidney tissue
Hub gene ELK3-mediated reprogramming lipid metabolism regulates phenotypic switching of pulmonary artery smooth muscle cells to develop pulmonary arterial hypertension induced by PM2.5 JOURNAL OF HAZARDOUS MATERIALS Qingping Liu WB Mouse 1:1000 right ventricle tissue
Potential crosstalk between SPP1 + TAMs and CD8 + exhausted T cells promotes an immunosuppressive environment in gastric metastatic cancer Journal of Translational Medicine Du Yan IF Mouse MFC cell-Xenograft
Maresin-1 ameliorates hypertensive vascular remodeling through its receptor LGR6 MedComm Zheng Yin IF,WB Mouse,Rat aorta tissue rat aortic smooth muscle cells (RASMCs)
Epigallocatechin gallate protects MC3T3-E1 cells from cadmium-induced apoptosis and dysfunction via modulating PI3K/AKT/mTOR and Nrf2/HO-1 pathways PeerJ Fanhao Wei WB Mouse MC3T3-E1 cell
Mussel-Derived and Bioclickable Peptide Mimic for Enhanced Interfacial Osseointegration via Synergistic Immunomodulation and Vascularized Bone Regeneration Advanced Science Wei Zhou IHC,WB Rat femur tissue bone marrow mesenchymal stem cell(BMSC)
Naringenin modulates the NO‑cGMP‑PKG signaling pathway by binding to AKT to enhance osteogenic differentiation in hPDLSCs INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Shenghong Li WB,IF Human,Rat 1:2000,1:100 periodontal ligament tissue,new bone tissue human periodontal ligament stem cell(hPDLSC)
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- Antibody-FAQs
- Products Images
- 4D Printing of Personalized-Tunable Biomimetic Periosteum with Anisotropic Microstructure for Accelerated Vascularization and Bone Healing. Mengfei Yu WB Human human mesenchymal stem cells (hMSCs)
- Western Blot analysis of various cells using OPN Polyclonal Antibody diluted at 1:500
- Western Blot analysis of 453 cells using OPN Polyclonal Antibody diluted at 1:500
- Western blot analysis of lysates from LOVO cells, using Osteopontin Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).